|Application ||IHC-P, IHC-F|
|Calculated MW||141479 Da|
|Immunogen||Multi drug resistant chinese hamster ovary cell line, CHrC5|
|Shelf Life||18 months from date of despatch.|
|Other Names||Multidrug resistance protein 1, 184.108.40.206, ATP-binding cassette sub-family B member 1, P-glycoprotein 1, CD243, ABCB1, MDR1, PGY1|
|Target/Specificity||Mouse anti Human CD243 antibody, clone JSB-1 recognizes human CD243, also known as Multidrug resistance protein 1 or P-glycoprotein-1 encoded by the ABCB1 gene. CD243 is a 1280 amino acid multi-pass membrane glycoprotein consisting of two homologous subunits bearing a hydrophobic N-terminal domain, six trans-membrane domains and a C-terminal hydrophilic domain containing a nucleotide binding site. The two sub-units are joined by a 60 amino acid linker domain (Hoet al.2003). CD243 has a predicted molecular mass of 141.5 kDa and apparent MW of ~190 kDa in Western blotting of human embryonal kidney cell line transfected with the MRP1 gene (Akanet al.2005) . CD243 is widely expressed being found in liver, kidney, small intestine and brain (Koepsell 1998).CD243 plays a critical role in the development of ‘multi-drug resistance’ during chemotherapy where it appears to control rated of efflux of chemotherapeutic drugs from cells (Scheperet al.1988). CD243 also appears to play a significant role on the development of gastrointestinal disease (Hoet al>2003), including conditions such as IBD13 or Inflammatory Bowel disease 13 (Walker-Smithet al.2009,Brantet al.2003).Mouse anti Human CD243 antibody, clone JSB-1 has been shown to detect CD243 by Western blotting in C5, a colchcine resistant CHO derived cell line, lysates (Jettéet al.1993) also in BeWo cell and placental trophoblast lysates(Ushigomeet al.2000) . Mouse anti human CD243 has also been used successfully for the detection of P-glycoprotein in teleost fish using immunohistochemistry (Hemmeret al.1995).|
|Preservative & Stabilisers||0.09% Sodium Azide, 0.5% Bovine Serum Albumin|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Human CD243 Antibody, clone JSB-1 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.