|Application ||IHC-P, IHC-F|
|Calculated MW||141479 Da|
|Immunogen||Multi drug resistant chinese hamster ovary cell line, CHrC5|
|Shelf Life||18 months from date of despatch.|
|Other Names||Multidrug resistance protein 1, 126.96.36.199, ATP-binding cassette sub-family B member 1, P-glycoprotein 1, CD243, ABCB1, MDR1, PGY1|
|Target/Specificity||Mouse anti Human CD243 antibody, clone JSB-1 recognizes human CD243, also known as Multidrug resistance protein 1 or P-glycoprotein-1 encoded by the ABCB1 gene. CD243 is a 1280 amino acid multi-pass membrane glycoprotein consisting of two homologous subunits bearing a hydrophobic N-terminal domain, six trans-membrane domains and a C-terminal hydrophilic domain containing a nucleotide binding site. The two sub-units are joined by a 60 amino acid linker domain (Hoet al.2003). CD243 has a predicted molecular mass of 141.5 kDa and apparent MW of ~190 kDa in Western blotting of human embryonal kidney cell line transfected with the MRP1 gene (Akanet al.2005) . CD243 is widely expressed being found in liver, kidney, small intestine and brain (Koepsell 1998).CD243 plays a critical role in the development of ‘multi-drug resistance’ during chemotherapy where it appears to control rated of efflux of chemotherapeutic drugs from cells (Scheperet al.1988). CD243 also appears to play a significant role on the development of gastrointestinal disease (Hoet al>2003), including conditions such as IBD13 or Inflammatory Bowel disease 13 (Walker-Smithet al.2009,Brantet al.2003).Mouse anti Human CD243 antibody, clone JSB-1 has been shown to detect CD243 by Western blotting in C5, a colchcine resistant CHO derived cell line, lysates (Jettéet al.1993) also in BeWo cell and placental trophoblast lysates(Ushigomeet al.2000) . Mouse anti human CD243 has also been used successfully for the detection of P-glycoprotein in teleost fish using immunohistochemistry (Hemmeret al.1995).|
|Preservative & Stabilisers||0.09% Sodium Azide, 0.5% Bovine Serum Albumin|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Human CD243 Antibody, clone JSB-1 is for research use only and not for use in diagnostic or therapeutic procedures.|
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