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Anti Human CD45 Antibody, clone F10-89-4

Mouse Anti-Human Monoclonal Antibody

     
  • FC - Anti Human CD45 Antibody, clone F10-89-4  ABD12901
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:FITC
  • FC - Anti Human CD45 Antibody, clone F10-89-4  ABD12901
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:RPE
  • FC - Anti Human CD45 Antibody, clone F10-89-4  ABD12901
    Published customer image:Mouse anti Human CD45 antibody, clone F10-89-4 used for the evaluation of CD45 expression on brain derived mesenchymal stem cells.Image caption:Brain-derived cell lines express markers for mesenchymal stem cells and pericytes but not for glial or neuronal precursors. (A) Representative histogram of flow cytometry analysis of cortical line and (B) ventricular zone line. Progenitors from all donors and both regions highly express MSC (CD90, CD73, CD105, CD29, CD166 and CD49d) and pericyte markers (CD140b/PDGFR-ß, RGS5, CD146, Nestin, a-SMA and NG2). They do not express hematopoietic , endothelial (CD31, CD34), microglial (CD14, CD11), glial or neuronal precursor cell markers (GFAP, O4) or myofibroblast markers , (green = isotype, red = respective marker).From: Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, et al. The Adult Human Brain Harbors Multipotent Perivascular Mesenchymal Stem Cells.PLoS ONE 7(4): e35577.
  • FC - Anti Human CD45 Antibody, clone F10-89-4  ABD12901
    Published customer image:Mouse anti Human CD45 antibody, clone F10-89-4 used for the evaluation of CD45 expression on brain derived mesenchymal stem cells.Image caption:FACS sorting of brain-derived progenitor cell lines. Human progenitor cells were positively sorted for the MSC markers CD105 and CD13, and thereafter negatively for hematopoietic and endothelial markers . Histogram illustrates results (A, B) for the two different cortical lines and (C, D) for the two lines from the ventricular zone. 99.4–100% of the cell population expressed both, CD105 and CD13, and cultures did not contain endothelial or hematopoietic cells.From: Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, et al. The Adult Human Brain Harbors Multipotent Perivascular Mesenchymal Stem Cells.PLoS ONE 7(4): e35577.
  • FC - Anti Human CD45 Antibody, clone F10-89-4  ABD12901
    Staining of human peripheral blood lymphocytes with Mouse anti HumanCD45:Alexa Fluor® 488
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, FC, IP
Primary Accession P08575
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names F10-89-4
Calculated MW 147254 Da
Additional Information
Purification Purified IgG prepared by ion exchange chromatography
Immunogen Human T lymphocytes.
Shelf Life 18 months from the date of despatch
Gene ID 5788
Other Names Receptor-type tyrosine-protein phosphatase C, 3.1.3.48, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45
Target/Specificity Mouse anti Human CD45 antibody, clone F10-89-4 recognizes the human CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc. Mouse anti Human CD45 antibody, clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. Mouse anti Human CD45 antibody, clone F10-89-4 is routinely tested in flow cytometry on human peripheral blood leucocytes
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4oC or at -20oC if preferred.
PrecautionsAnti Human CD45 Antibody, clone F10-89-4 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12901
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