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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti Human Macrophages Antibody, clone MAC387    

Anti Human Macrophages Antibody, clone MAC387

Mouse Anti-Human Monoclonal Antibody

     
  • IHC-P - Anti Human Macrophages Antibody, clone MAC387  ABD12902
    Published customer image:Mouse anti Human macrophages antibody, clone MAC387 used for the identification of macrophages in canine skin by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Bites of Lu. longipalpis sand flies induce a strong focal and systemic adaptive cellular immune response in dogs immunized with LJL143 or LJM17.(A–C) Dogs were exposed to uninfected and infected sand flies for 10 min one month after the final immunization with either LJM17, LJL143 or the empty plasmid . (A–C) Skin biopsies obtained from bite sites 48 h post challenge with 20 and five uninfected and 10 infected sand flies were cut in half and processed for histology and RNA extraction. (A) Representative H&E staining and immunohistochemical labeling of T cells and macrophages at the bite sites of 20 uninfected sand flies in LJL143- and LJM17-immunized and control dogs. (B) Reverse-transcriptase quantitative PCR showing the expression levels of IFN-?, IL-12, IL-4 and TGF-ß at the bite sites of 20 or five uninfected sand flies in LJL143- and LJM17-immunized and control dogs (for control dogs RNA was combined from sites of 20 and 5 uninfected sand fly bites). (C) Same as (B) using 10 infected sand flies. Histological sections from bite sites of five uninfected and 10 infected sand flies are provided as Figure S1 and Figure S2, respectively. (D–E) PBMC from LJL143- and LJM17-immunized and control dogs obtained one week after exposure to sand flies. (D) Frequency and mean fluorescence intensity of CD3+ T cells following stimulation with medium, rLJL143 or rLJM17. (E) Frequency of CD4+ and CD8+ T cells expressing IFN-? in PBMC from LJL143- and LJM17-immunized dogs. Error bars represent means±S.E. * P
  • IHC - Anti Human Macrophages Antibody, clone MAC387  ABD12902
    Published customer image:Mouse anti Human macrophages antibody, clone MAC387 used for the identification of macrophages in canine skin by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Bites of Lu. longipalpis sand flies induce a strong focal cellular immune response in dogs immunized with LJL143 or LJM17. Dogs were exposed to 5 uninfected sand flies for 10 min one month after the final immunization with either LJM17, LJL143 or empty plasmid . Skin biopsies obtained from bite sites 48 h post challenge were processed for histology. Representative H&E staining and immunohistochemical labeling of T cells and macrophages at the bite sites in LJL143- and LJM17-immunized and control dogs.From: Collin N, Gomes R, Teixeira C, Cheng L, Laughinghouse A, et al. Sand Fly Salivary Proteins Induce Strong Cellular Immunity in a Natural Reservoir of Visceral Leishmaniasis with Adverse Consequences for Leishmania. PLoS Pathog 5(5): e1000441.
  • IHC - Anti Human Macrophages Antibody, clone MAC387  ABD12902
    Published customer image:Mouse anti Human macrophage antibody, clone MAC387 used for the detection of macrophages in rabbit skin by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Comparison of Regranex and ATP-vesicle treated wounds 3 days postoperatively. Rich collections of macrophages are stained dark brown to black by Anti-Mac387 (top panels), and more collagen is shown by van Gieson stain (low panels) in the wounds treated with ATP-vesicles. Regranex treated wounds do not show a similar effect.From: Howard JD, Sarojini H, Wan R, Chien S Rapid Granulation Tissue Regeneration by Intracellular ATP Delivery-A Comparison with Regranex. PLoS ONE 9(3): e91787.
  • FC - Anti Human Macrophages Antibody, clone MAC387  ABD12902
    Staining of human peripheral blood granulocytes with FITC conjugated Mouse anti Human macrophages following permeabilization with Leucoperm
  • IHC - Anti Human Macrophages Antibody, clone MAC387  ABD12902
    Published customer image:Mouse anti Human macrophages antibody, clone MAC387 used for the identification of macrophages in canine skin by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Bites of L. i. chagasi infected sand flies induce a strong focal cellular immune response in dogs immunized with LJL143 or LJM17. Dogs were exposed to ten L. i. chagasi infected sand flies for 10 min one month after the final immunization with either LJM17, LJL143 or empty plasmid . Skin biopsies obtained from bite sites 48 h post challenge were processed for histology. Representative H&E staining and immunohistochemical labeling of T cells and macrophages at the bite sites in LJL143- and LJM17-immunized and control dogs.From: Collin N, Gomes R, Teixeira C, Cheng L, Laughinghouse A, et al. Sand Fly Salivary Proteins Induce Strong Cellular Immunity in a Natural Reservoir of Visceral Leishmaniasis with Adverse Consequences for Leishmania. PLoS Pathog 5(5): e1000441.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, FC
Primary Accession P06702
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names MAC387
Calculated MW 13242 Da
Additional Information
Other Species Hs,Pig,Dog,Rb,Baboon,B,Fallow deer,Pygmy hippopotamus,Mink,Gp,Rat,Cat,Cynomolgus monkey,Rh,Ga
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Human monocytes.
Shelf Life 18 months from the date of despatch
Gene ID 6280
Other Names Protein S100-A9, Calgranulin-B, Calprotectin L1H subunit, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, MRP-14, p14, S100 calcium-binding protein A9, S100A9, CAGB, CFAG, MRP14
Target/Specificity Mouse anti Human macrophages, clone MAC387 recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. Although originally described as binding to epitopes common to both the alpha and beta chains (Flavellet al.1987) subsequent studies indicate that the antibody detects an epitope exclusively expressed on the beta chain (Goebeleret al.1994) demonstrated by immunofluorescent and western blotting on both naturally expressing and transfected targets. In addition Mouse anti Human macrophages, clone MAC387 detects the beta chain in complex with the alpha.The antigen recognized by Mouse anti Human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cellds in a wide range of species.
Preservative & Stabilisers None present
Storage Store at -20℃ only.
PrecautionsAnti Human Macrophages Antibody, clone MAC387 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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