|Application ||IHC-F, IF, FC|
|Calculated MW||182979 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Purified murine sialoadhesin.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Sialoadhesin, Sheep erythrocyte receptor, SER, Sialic acid-binding Ig-like lectin 1, Siglec-1, CD169, Siglec1, Sa, Sn|
|Target/Specificity||Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also known as sialoadhesin, Sheep erythrocyte receptor or Siglec-1. CD169 is a 1695 amino acid, ~180 kDa single pass, type 1 transmembrane glycoprotein containing a single Ig-like V-type domain and sixteen Ig-like C2-type domains. CD169 is a macrophage restricted receptor, preferentially binding to alpha 2,3 linked sialic acid residues (Crockeret al.1991) and is expressed on stromal macrophages in many tissues, particularly in lymph nodes, bone marrow and on marginal metallophilic macrophages in the spleen (Morriset al.1991).CD169 has been implicated in a number of roles including cell-cell interactions with lymphocytes (van den Berg et al.1992) and granulocytes (Crockeret al.1995). CD169 expressing macrophages have also been suggested to play a role in host resistance to lymphoma metastasis (Umanskyet al.1996). In pigs CD169 has also been identified as a macrophage restricted receptor for porcine reproductive and respiratory syndrome virus (Delputteet al.2007). CD169 expressing macrophages have also been implicated in the regulation of autoimmune disease progression through their interaction with regulatory T cells via CD169 (Wuet al.2009). CD169 has also been shown to play a critical role in the recognition and elimination of invasive sialylated microorganisms including Campylobacter jejuni(Klasset al.2012) and group B Streptococcus (Changet al.2014).The functional activity of rat anti mouse CD169 antibody, clone 3D6.112, its ability to inhibit binding of red blood cells to CD169 can be considerably enhanced by derivitization of the antibody with polyethylene glycol (Ducreuxet al.2008).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4oC or at -20oC if preferred.|
|Precautions||Anti Mouse CD169 antibody, clone 3D6.112 is for research use only and not for use in diagnostic or therapeutic procedures.|
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