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>   home   >   Products   >   Primary Antibodies   >   Anti Mouse CD169 antibody, clone 3D6.112   

Anti Mouse CD169 antibody, clone 3D6.112

Rat Anti-Mouse Monoclonal Antibody

     
  • IF - Anti Mouse CD169 antibody, clone 3D6.112 ABD12905
    Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power
  • IF - Anti Mouse CD169 antibody, clone 3D6.112 ABD12905
    Published customer image:Rat anti Mouse CD169 antibody, clone 3D6.112 used for the detection of sialoadhesin im mouse spleen by immunofluorescence.Image caption:Splenic marginal zone macrophages and marginal metallophilic macrophages control L. donovani by an IFN-? independent mechanism. (A) L. donovani amastigotes are found predominantly in MMM and MZM after intravenous injection (purple, SIGNR1; green CD169; white, L. donovani; blue DAPI). (B) C57BL/6 mice were treated with 0.5mg XMG 1.6 anti-IFN-? IgG (black bars) or control IgG (open bars) and 2h later infected with L. donovani. At 5h and 24h p.i., the number of parasites per MZ area was determined. (C) 14M1.4 cells (black bars) and RAW 264.7 cells (open bars) were infected with L. donovani (MOI 10:1) and at indicated times p.i., parasite numbers per 100 macrophages were determined by fluorescence microscopy. *, p
  • IF - Anti Mouse CD169 antibody, clone 3D6.112 ABD12905
    Published customer image:Rat anti Mouse CD169 antibody, clone 3D6.112 used for the detection of sialoadhesin im mouse spleen by immunofluorescence.Image caption:IRF-7 is induced in MZM and MM in situ. (A–D) Induction of IRF-7 in MMM and MZM. Naïve (A,B) and C57BL/6 mice infected for 24h (C, D) were stained for CD169 (A, C; green) or SIGNR1 (B, D; green), IRF-7 and L. donovani . (E) The percentage of IRF-7+ parasite-containing phagosomes was quantified by counting 100 amastigotes/section (n = 3) and calculating the proportion of parasites with IRF-7 accumulation. *, p
  • IHC - Anti Mouse CD169 antibody, clone 3D6.112 ABD12905
    Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Low power
  • IHC - Anti Mouse CD169 antibody, clone 3D6.112 ABD12905
    Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. High power
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-F, IF, FC
Primary Accession Q62230
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names 3D6.112
Calculated MW 182979 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Purified murine sialoadhesin.
Shelf Life 18 months from date of despatch.
Gene ID 20612
Other Names Sialoadhesin, Sheep erythrocyte receptor, SER, Sialic acid-binding Ig-like lectin 1, Siglec-1, CD169, Siglec1, Sa, Sn
Target/Specificity Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also known as sialoadhesin, Sheep erythrocyte receptor or Siglec-1. CD169 is a 1695 amino acid, ~180 kDa single pass, type 1 transmembrane glycoprotein containing a single Ig-like V-type domain and sixteen Ig-like C2-type domains. CD169 is a macrophage restricted receptor, preferentially binding to alpha 2,3 linked sialic acid residues (Crockeret al.1991) and is expressed on stromal macrophages in many tissues, particularly in lymph nodes, bone marrow and on marginal metallophilic macrophages in the spleen (Morriset al.1991).CD169 has been implicated in a number of roles including cell-cell interactions with lymphocytes (van den Berg et al.1992) and granulocytes (Crockeret al.1995). CD169 expressing macrophages have also been suggested to play a role in host resistance to lymphoma metastasis (Umanskyet al.1996). In pigs CD169 has also been identified as a macrophage restricted receptor for porcine reproductive and respiratory syndrome virus (Delputteet al.2007). CD169 expressing macrophages have also been implicated in the regulation of autoimmune disease progression through their interaction with regulatory T cells via CD169 (Wuet al.2009). CD169 has also been shown to play a critical role in the recognition and elimination of invasive sialylated microorganisms including Campylobacter jejuni(Klasset al.2012) and group B Streptococcus (Changet al.2014).The functional activity of rat anti mouse CD169 antibody, clone 3D6.112, its ability to inhibit binding of red blood cells to CD169 can be considerably enhanced by derivitization of the antibody with polyethylene glycol (Ducreuxet al.2008).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4oC or at -20oC if preferred.
PrecautionsAnti Mouse CD169 antibody, clone 3D6.112 is for research use only and not for use in diagnostic or therapeutic procedures.
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