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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti Human Cytokeratin 14 Antibody, clone LL002    

Anti Human Cytokeratin 14 Antibody, clone LL002

Mouse Anti-Human Monoclonal Antibody

     
  • IHC-P - Anti Human Cytokeratin 14 Antibody, clone LL002  ABD12908
    Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human cytokeratin 14 followed by HRP polymer detection and DAB substrate development (high power) following heat mediated antigen retrieval using citrate buffer at pH6.2
  • IHC - Anti Human Cytokeratin 14 Antibody, clone LL002  ABD12908
    Published customer image:Mouse anti Human cytokeratin 14 antibody, clone LL002 used for the detection of CK14 expressing cells in a canine inflammatory mammary cancer pellet section by immunohistochemistry.Image caption:Immunohistochemistry of IPC-366 pellet, paraffin sections. A) Pancytokeratin (AE1/AE3) ; intense immunolabeling and numerous positive cells. B) CK14; intense immunolabeling and numerous positive cells . C) Vimentin; moderate immunolabeling and numerous positive cells . D) p63; negative . E) a-smooth muscle actin, negative cells . F) Estrogen receptor; negative cells . G) Progesterone receptor; negative cells . H) HER-2; negative cells . I) E-Cadherin; intense membranous immunolabeling in numerous cells . J) COX-2; most of the cells are moderately positive; some cells are intensely positive . K) COX-2; some cells strongly positive showing features of malignancy (binucleated cell, arrow) . L) Ki-67 proliferation marker; many nuclei are positive .From: Caceres S, Peña L, de Andres PJ, Illera MJ, Lopez MS, et al. Establishment and Characterization of a New Cell Line of Canine Inflammatory Mammary Cancer: IPC-366. PLoS ONE 10(3): e0122277.
  • FC - Anti Human Cytokeratin 14 Antibody, clone LL002  ABD12908
    Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human cytokeratin 14 followed by HRP polymer detection and DAB substrate development (low power) following heat mediated antigen retrieval using citrate buffer at pH6.2
  • IHC - Anti Human Cytokeratin 14 Antibody, clone LL002  ABD12908
    Immunoperoxidase staining of formalin fixed, paraffin embedded human tonsil with Mouse anti Human cytokeratin 14 folllowing heat mediated antigen retrieval using citrate buffer, pH6.0
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, FC
Primary Accession P02533
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG3
Clone Names LL002
Calculated MW 51561 Da
Additional Information
Other Species Elephant,Dog,Pig,Lion
Purification Purified IgG prepared by ion exchange chromatography
Immunogen Last 15 C-terminal residues of human cytokeratin 14 conjugated to thyroglobulin.
Shelf Life 18 months from date of despatch.
Gene ID 3861
Other Names Keratin, type I cytoskeletal 14, Cytokeratin-14, CK-14, Keratin-14, K14, KRT14
Target/Specificity Mouse anti Human Cytokeratin 14 antibody, clone LL002 recognizes cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express cytokeratin 14.Mouse anti cytokeratin 14, clone LL002 has been reported to be suitable for use in Western blotting (Alamet al.2011)
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human Cytokeratin 14 Antibody, clone LL002 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12908
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