|Application ||WB, FC|
|Calculated MW||28147 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Shelf Life||18 months from date of despatch.|
|Other Names||Lymphocyte function-associated antigen 3, Ag3, Surface glycoprotein LFA-3, CD58, CD58, LFA3|
|Target/Specificity||Mouse anti Human CD58 antibody, clone BRIC5 recognizes human Lymphocyte function-associated antigen 3, also known as CD58 or LFA-3. CD58 is a 250 amino acid single pass type I transmembrane glycoprotein, a member of the immunoglobulin superfamily, with a predicted molecular mass of 28.1 kDa and an apparent molecular mass of ~55-70 kDa. CD58 occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA and is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes (Makgobaet al.1989).CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.Mouse anti Human CD58 antibody, clone BRIC5 was produced in response to erythrocytes. The functional affinity of BRIC5 binding to erythrocytes is 4 x 108M-1. It reacts by immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect haemagglutinin. The antigen on erythrocytes is pronase sensitive. BRIC5 inhibits T cell rosetting.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Human CD58 Antibody, clone BRIC5 is for research use only and not for use in diagnostic or therapeutic procedures.|
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