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>   home   >   Products   >   Primary Antibodies   >   Anti Mouse Dendritic/Interdig. Cells Antibody, clone MIDC-8    

Anti Mouse Dendritic/Interdig. Cells Antibody, clone MIDC-8

Rat Anti-Mouse Monoclonal Antibody

  • FC - Anti Mouse Dendritic/Interdig. Cells Antibody, clone MIDC-8  ABD12924
    Published customer image:Rat anti Mouse dendritic/interdigitating cells antibody, clone MIDC-8 used for the detection of dendritic cells by immunohistochemistry on cryostat sections.Image caption:Loss of MHC class II expression and bone-marrow-derived stromal cell elements in the thymus of IL-2-/- mice. Frozen sections of thymuses from 5-week-old SPF IL-2+/+ (+/+) and IL-2 -/- (-/-) mice were stained with the anti-macrophage-specific antibodies Mac-1 (A and B) and F4/80 (C and D), and with the anti-thymic dendritic-cell antibody MIDC-8 (E and F) and the anti-MHC class II-specific antibody Y-3P (G and H). Bound antibody was visualized as described in the legend to Fig. 4. The arrow heads in E and F identify positively stained dendritic cells, m: medulla; c: cortex. Magnification: 200.From: Tannishtha Reya, Hamid Bassiri, Renée Biancaniello, and Simon R. Carding, “Thymic Stromal-Cell Abnormalities and Dysregulated T-Cell Development in IL-2-Deficient Mice,” Developmental Immunology, vol. 5, no. 4, pp. 287-302, 1998.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names MIDC-8
Additional Information
Purification Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Immunogen Mouse lymph node stroma.
Shelf Life 18 months from date of despatch.
Target/Specificity Rat anti Mouse dendritic/interdigitating cells antibody, clone MIDC-8 recognizes an antigen in the cytoplasm of rat dendritic cells as distinct spots near the nucleus.Rat anti Mouse dendritic/interdigitating cells antibody, clone MIDC-8 does not stain thymic epithelial cells which are the major contaminants in thymic dendritic cell preparations. The antigen is also absent in blood cells, bone marrow cells and macrophages isolated from the peritoneal cavity. The antigen recognized by Rat anti Mouse dendritic/interdigitating cells antibody, clone MIDC-8 is found in interdigitating cells in T cell dependent areas of secondary lymphoid organs, the medulla of thymus, veiled cells, and very weakly in Langerhans cells (Breelet al.1987).
Preservative & Stabilisers 0.09%Sodium Azide
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Mouse Dendritic/Interdig. Cells Antibody, clone MIDC-8 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12924
(40 western blots)
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