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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti Mouse CD205 Antibody, clone NLDC-145    

Anti Mouse CD205 Antibody, clone NLDC-145

Rat Anti-Mouse Monoclonal Antibody

  • FC - Anti Mouse CD205 Antibody, clone NLDC-145  ABD12925
    Staining of mouse thymus cells with Rat anti Mouse CD205:FITC
  • IHC - Anti Mouse CD205 Antibody, clone NLDC-145  ABD12925
    Immunoperoxidase staining of cryosectioned mouse lymph node with Rat anti Mouse CD205
  • IHC - Anti Mouse CD205 Antibody, clone NLDC-145  ABD12925
    Immunoperoxidase staining of mouse dendritic cells with Rat anti Mouse CD205
  • IF - Anti Mouse CD205 Antibody, clone NLDC-145  ABD12925
    Immunofluorescent staining of Langerhans cells in mouse skin with Rat anti Mouse CD205
  • IHC - Anti Mouse CD205 Antibody, clone NLDC-145  ABD12925
    Published customer image:AIRE expression in the unreverted R/-; CreERt2 thymic rudiment. 3–4 months old R/-; CreERt2 mice were injected with 4OHT at the doses shown (A, carrier-only, B, 0.25 mg 4OHT, C, 2 mg 4OHT). Images show immunohistochemical analysis of thymi or thymic rudiments from 4OHT mice 7 weeks post-injection. Staining is shown for AIRE, counterstained for cytokeratin 8 or CD205 as shown. DAPI reveals nuclei . (A) Top and bottom panels show representative images of AIRE- regions (which comprised the majority of sections), and a rare AIRE+ cell, respectively. (B) Top and middle panels show representative images of AIRE- regions and AIRE+ cells, respectively. Bottom panel shows higher power image of middle panel. Carrier-only and 0.25 mg 4OHT injected mice, n = 2; reverted mice (i.e. injected with =0.5 mg 4OHT), n>3 (shown for 2 mg injected). Scale bars 100 µm except where shown.From: Jin X, Nowell CS, Ulyanchenko S, Stenhouse FH, Blackburn CC Long-Term Persistence of Functional Thymic Epithelial Progenitor Cells In Vivo under Conditions of Low FOXN1 Expression. PLoS ONE 9: e114842.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q60767
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names NLDC-145
Calculated MW 197352 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Mouse lymph node stroma.
Shelf Life 18 months from date of despatch.
Gene ID 17076
Other Names Lymphocyte antigen 75, Ly-75, DEC-205, CD205, Ly75, Cd205
Target/Specificity Rat anti Mouse CD205, clone NLDC-145 recognizes DEC-205, a ~205 kDa integral membrane glycoprotein expressed by mouse dendritic cells and thymic epithelial cells. It is absent in bone marrow cells, all blood cells and freshly isolated macrophages from the peritoneal cavity. A subpopulation of the latter becomes positive in mice previously stimulated with thioglycollate. It is also absent on follicular dendritic cells of B cell follicles.The antigen is found on la positive interdigitating cells in T cell areas of all secondary lymphoid organs. It is also present on veiled cells. In non-lymphoid organs, the antigen is only found on Langerhans cells of the skin but cross reactions with la positive thymic epithelial cells and intestinal villus epithelial cells are also observed.DEC-205 is expressed at the cell surface of unfixed cells but can also be found in the cytoplasm of fixed cells.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Mouse CD205 Antibody, clone NLDC-145 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12925
(40 western blots)
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