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Anti Rat Granulocytes and Erythroid Cells Antibody, clone HIS48

Mouse Anti-Rat Monoclonal Antibody

  • IF - Anti Rat Granulocytes and Erythroid Cells Antibody, clone HIS48  ABD12931
    Published customer image:Mouse anti Rat granulocytes and erythroid cells antibody, clone HIS48 used for stauiining granulocytes in rat corneal sections by immunofluorescence.Image caption:Immunofluorescence images on flat-mounted corneas in a model of endotoxin-induced uveitis analyzed with a 3D laser confocal microscope with a 20X enlargement. Immunostainings with anti-CD68 for macrophages (A), anti-TCR alpha/beta for lymphocytes (B), anti-MCA967 for granulocytes (C), and IgG1 mouse antibody for isotypic control (D) were revealed with secondary antibody, Alexa Fluor (in green) while the nuclear chromatin was stained with propidium iodide (in red). A double immunostaining with Alexa Fluor 488 phalloidin (in green), and antibody to CD68 (in red) was performed to localize the macrophages among the corneal layers, notably on the endothelium (E).From: Trinh L, Brignole-Baudouin F, Labbé A, Raphaël M, Bourges JL, Baudouin C. The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry.Mol Vis. 2008 Jun16;14:1149-56.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgM
Clone Names HIS48
Additional Information
Purification Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 8% foetal calf serum
Immunogen PVG rat spleen cell suspension.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti Rat granulocytes and erythroid cells antibody, clone HIS48 recognizses granulocytes and erythroid cells.Clone HIS48 has frequently been used to stain rat neutrophils in immunohistochemistry (Recklesset al.2001).
Preservative & Stabilisers 0.09%Sodium Azide
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Rat Granulocytes and Erythroid Cells Antibody, clone HIS48 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12931
(40 western blots)
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