Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Anti Rat RECA-1 Antibody, clone HIS52    

Anti Rat RECA-1 Antibody, clone HIS52

Mouse Anti-Rat Monoclonal Antibody

     
  • IF - Anti Rat RECA-1 Antibody, clone HIS52  ABD12932
    Published customer image:Mouse anti Rat RECA-1 antibody, clone HIS52 used for the detection of RECA-1 expressing cells by immunofluorescence.Image captionhADSCs led to the appearance of perivascular spaces in between endothelial and astrocytic basement membranes one week after injection. A–D) Confocal images of horizontal sections immunostained with anti-pan-laminin antibody one week after injury. Note that in DMEM animals (A,B) there is no separation between the two membranes whereas in hADSCs–treated animals (C,D) these membranes are separated (arrows in D). E) Confocal images of a horizontal section immunostained with anti-pan-laminin and RECA-1. F–F”) Confocal imagens of sequential optical sections immunostained with anti-pan-laminin and DAPI showing the extravasation of cells from the blood vessels. Bars: C, F = 50 µm B, D, E = 25 µm.From: Menezes K, Nascimento MA, Gonçalves JP, Cruz AS, Lopes DV, et al. Human Mesenchymal Cells from Adipose Tissue Deposit Laminin and Promote Regeneration of Injured Spinal Cord in Rats. PLoS ONE 9(5): e96020.
  • IF - Anti Rat RECA-1 Antibody, clone HIS52  ABD12932
    Published customer image:Mouse anti Rat RECA-1 antibody, clone HIS52 used for the detection of RECA-1 expressing cells by immunofluorescence.Image captionEffect of clustered ephrin-A1-Fc on vascular formation in the rat striatum. (A) Distribution of BrdU(+) endothelial cells. Brain of the unilaterally lesioned rats 6 weeks after infusion of clustered ephrin-A1-Fc were sectioned coronally and stained for BrdU and Rat Endothelial Cell Antigen-1 (RECA-1). Numbers of the BrdU(+) cells and BrdU(+)&RECA-1(+) cells were counted as describe in the Materials and Methods. Total numbers of BrdU(+) cells in 8 animals are shown on the top, and percentages of RECA-1(+) cells among BrdU(+) cells are shown on the bottom. Error bars represent SD. *p
  • IF - Anti Rat RECA-1 Antibody, clone HIS52  ABD12932
    Published customer image:Mouse anti Rat RECA-1 antibody, clone HIS52 used for the detection of RECA-1 expressing cells by immunofluorescence.Image captionChABC and GF treatments attenuate the proliferation of microglia/macrophages and promote the generation of new endothelial cells after SCI. (A–D) Representative confocal images of BrdU+/NG2+ macrophages/microglia marked with Iba-1 in the injured spinal cord . (E–F) Under baseline SCI condition, macrophages/microglia comprised about 25% and 17% of BrdU+/NG2+ cells in rostral and caudal points to the injury center, respectively. After treatment with ChABC and/or GFs, we found a reduction in the number of BrdU+/NG2+/IbA-1+ cells that was statistically significant for ChABC and ChABC+GFs treatment groups relative to the vehicle group. (G–J) Representative confocal images show newly generated endothelial cells marked by Reca-1 and NG2 among BrdU+ cells. Reca-1 positive endothelial cells comprised a subpopulation of proliferating NG2+ cells after SCI (J). (K–L) Quantification of BrdU+/NG2+/Reca-1+ cells showed a significant number of newly generated endothelial cells after treatment with ChABC and/or GFs at both rostral and caudal points to the injury center compared to the vehicle group. *p
  • IF - Anti Rat RECA-1 Antibody, clone HIS52  ABD12932
    Published customer image:Mouse anti Rat RECA-1 antibody, clone HIS52 used for the detection of RECA-1 expressing cells in rat kidney by immunofluorescence.Image caption:Low magnification immunofluorescence staining for RECA-1, HGF, IGF-1 and Pax-2 on male MWF renal tissues.Sections are labeled with WGA-lectin and DAPI . Dashed lines divide the graft from the host, while dotted lines divide the area adjacent to and distant from the graft. Scale bars = 100 µm.From: Imberti B, Corna D, Rizzo P, Xinaris C, Abbate M, et al. Renal Primordia Activate Kidney Regenerative Events in a Rat Model of Progressive Renal Disease.PLoS ONE 10(3): e0120235.
  • IF - Anti Rat RECA-1 Antibody, clone HIS52  ABD12932
    Published customer image:Mouse anti Rat RECA-1 antibody, clone HIS52 used for the detection of RECA-1 expressing cells by immunofluorescence.Image captionMorphology of microvessels stained with RECA-1 in Aß 1-42-injected hippocampus. Panels show morphological features including fragments, looping microvessels, and vessels with knob-like and uneven diameters. The scale bar represents 40? µm.From: Jantaratnotai N, Ryu JK, Schwab C, McGeer PL, McLarnon JG. Comparison of Vascular Perturbations in an Aß-Injected Animal Model and in AD Brain. Int J Alzheimers Dis. 2011;2011:918280.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-F, IF
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names HIS52
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Stromal cells from rat lymph node.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti Rat RECA-1 antibody, clone HIS52 recognizes RECA-1, a cell surface antigen which is expressed by all rat endothelial cells. The endothelium is the thin layer of cells that line the interior surface of blood vessels, forming an interface between circulating blood in the lumen and the rest of the vessel wall. Endothelial cells line the entire circulatory system, from the heart to the smallest capillary.
Preservative & Stabilisers 0.09%Sodium Azide
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Rat RECA-1 Antibody, clone HIS52 is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to
info@abgent.com, and receive a free "I Love Antibodies" mug.
FeedBack
Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

Cat# ABD12932
Size:
Quantity:
Availability: Inquire
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
30% off on 400+ Stem Cells Antibodies. PromoCode: <span class=text-red>FLASH19</span>
Terms & Conditions