Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Anti BrdU Antibody, clone BU1/75 (ICR1)    

Anti BrdU Antibody, clone BU1/75 (ICR1)

  • IF - Anti BrdU Antibody, clone BU1/75 (ICR1)  ABD12938
    Published customer image:Rat anti BrdU antibody, clone Bu1/75 used for the detection of BrdU positive cells in mouse brain by immunofluorescence.Image caption:Disruption of the Stx1 function impairs GBM tumor progression in vivo. An equal number of the indicated U373 cell populations were stereotactically inoculated into the brain of athymic nude mice. The size of the tumors was estimated at different days post-inoculation by the quantification of luciferase activity in the tumor cells. A, D. Representative images of the luciferase signal from mice inoculated with the indicated U373 GBM cells after 40 DPI. B, E. Growth curves of the indicated U373 GBM tumors showing a marked reduction of the tumor sizes after impairment of Stx1 function (* p = 0.05 at 40 DPI). C, F. Scatter plots showing the individual size of the indicated U373 GBM tumors after 40 DPI. G. Representative entire brain NMR images of mice inoculated with the indicated U373 cell populations after 30 DPI (arrowheads indicate the tumors formed) showing a marked reduction in the Stx1a-Sh01 and Stx1-DN cells. H. Scatter plot showing the area of the indicated U373 GBM tumors formed after 30 DPI. I. Representative confocal images from histological sections of 30 DPI brain tumors stained with anti-GFP and anti-BrdU antibodies. J. Quantification of BrdU-positive nuclei in 30 DPI U373 cell tumors showing that Stx1 loss-of-function reduces proliferation (* p = 0.05; *** p = 0.001).From: Ulloa F, Gonzàlez-Juncà A, Meffre D, Barrecheguren PJ, Martínez-Mármol R, et al. Blockade of the SNARE Protein Syntaxin 1 Inhibits Glioblastoma Tumor Growth.PLoS ONE 10(3): e0119707.
  • IHC - Anti BrdU Antibody, clone BU1/75 (ICR1)  ABD12938
    Published customer image:Rat anti BrdU antibody used for the detection of BrdU positive cells in Mouse brain by immunohistochemistry on cryosections.Image caption:PEDF Treatment Does Not Affect Cell Proliferation After Striatal Ischemic Injury. Mice received daily injections of 50 mg/kg BrdU for seven days starting at the day of MCAO. (A) Representative images of cortex, striatum, subventricular zone , and dentate gyrus of CSF and PEDF group. The dotted line in the images of SVZ indicates the area used for counting the cells (distance of 50 µm from the ventricles). Scale bar = 200 µm. (B–C) Graphs represent the number of BrdU-positive cells/mm2 in striatum, cortex, SVZ, and dentate gyrus. We did not find any significant differences between CSF and PEDF groups. (B) We found a significant difference between ipsilateral and contralateral striatum for both PEDF (repeated measures ANOVA, F(1,7) = 76.994, p
  • IHC - Anti BrdU Antibody, clone BU1/75 (ICR1)  ABD12938
    Published customer image:Rat anti BrdU antibody, clone Bu1/75 used for the identification of newly divided cells in rat jejenum by immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Image caption:BrdU labeling of the small intestine after i.v. administration. BrdU (50 mg/kg b.wt.), a thymidine analogue, was given 2 h prior to removing the intestine. The dark labeling by BrdU is exclusively located to the nuclei of intestinal crypt cells. Mayer's haematoxylin.From: Lundgren O, Jodal M, Jansson M, Ryberg AT, Svensson L. Intestinal epithelial stem/progenitor cells are controlled by mucosal afferent nerves.PLoS One. 2011 Feb 9;6(2):e16295.
  • IHC-P - Anti BrdU Antibody, clone BU1/75 (ICR1)  ABD12938
    Formalin fixed, paraffin embedded mouse colon stained with Rat anti BrdU: med power
  • IHC-P - Anti BrdU Antibody, clone BU1/75 (ICR1)  ABD12938
    Formalin fixed, paraffin embedded mouse colon stained with Rat anti BrdU: low power
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names BU1/75 (ICR1)
Additional Information
Other Species Axolotl
Purification Purified IgG prepared by affinity chromatography on Protein G
Shelf Life 18 months from date of despatch.
Target/Specificity Rat anti BrdU antibody clone BU1/75 (ICR1), recognizes bromodeoxyuridine (known as BrdU or BrdUrd). Rat anti BrdU antibody clone BU1/75 (ICR1) reacts with BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU.Rat anti BrdU antibody, clone BU1/75 (ICR1) cross reacts with chlorodeoxyuridine (CldU) but does not cross react with thymidine or iododeoxyuridine (Atenet al.1992). BrdU, IdU and CldU are analogs of thymidine, they can incorporate into DNA during DNA synthesis replacing thymidine.Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivoandin vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqziet al.2004) or in drinking water (Moseret al.2004)Clone BU1/75 (ICR1) has been used to detect CldU to study the speed of DNA replication fork (Bugleret al.2010) , in the detection of CldU label retaining stem cells (Kimotoet al.2008) and label retaining neurons (Murataet al.2011).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
PrecautionsAnti BrdU Antibody, clone BU1/75 (ICR1) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.
Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

Cat# ABD12938
(40 western blots)
Availability: Inquire
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Santa Cruz Alternative
Terms & Conditions