|Application ||IHC-P, IF, FC|
|Clone Names||BU1/75 (ICR1)|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Rat anti BrdU antibody clone BU1/75 (ICR1), recognizes bromodeoxyuridine (known as BrdU or BrdUrd). Rat anti BrdU antibody clone BU1/75 (ICR1) reacts with BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU.Rat anti BrdU antibody, clone BU1/75 (ICR1) cross reacts with chlorodeoxyuridine (CldU) but does not cross react with thymidine or iododeoxyuridine (Atenet al.1992). BrdU, IdU and CldU are analogs of thymidine, they can incorporate into DNA during DNA synthesis replacing thymidine.Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivoandin vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqziet al.2004) or in drinking water (Moseret al.2004)Clone BU1/75 (ICR1) has been used to detect CldU to study the speed of DNA replication fork (Bugleret al.2010) , in the detection of CldU label retaining stem cells (Kimotoet al.2008) and label retaining neurons (Murataet al.2011).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Precautions||Anti BrdU Antibody, clone BU1/75 (ICR1) is for research use only and not for use in diagnostic or therapeutic procedures.|
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