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>   home   >   Products   >   Primary Antibodies   >   Anti Hepatitis B Surface Antigen Antibody    

Anti Hepatitis B Surface Antigen Antibody

Rabbit Anti-Viral Polyclonal Antibody

  • WB - Anti Hepatitis B Surface Antigen Antibody  ABD12988
    Published customer image:Rabbit anti hepatitis B surface antigen antibody used for the evaluation of hepatitis B surface antigen in infected cell cultures by western blottingImage caption:(A) Materials released from Vero/hSLAM cells infected with MVvac2DIII-S,S)P or MVvac2N collected 72 h after infection were clarified, and particulate material pelleted, loaded on a 20% to 60% sucrose gradient, and centrifuged to equilibrium. Five hundred microliter fractions were collected from the top to the bottom and weighed. The dotted line shows a representative density profile. (B) Aliquots of each fraction were separated by 12.5% SDS-polyacrylamide gel electrophoresis, immunoblotted, and probed with anti-HBsAg antibodies for fractions coming from cells infected with MVvac2DIII-S,S)P (upper panel) or MVvac2N (medium panel). The lower panel indicates the migration of infectious MV virions.From: Immunogenic Subviral Particles Displaying Domain III of Dengue 2 Envelope Protein Vectored by Measles Virus.Harahap-Carrillo, I.S. et al.Vaccines 2015; 3(3), 503-18. doi:10.3390/vaccines3030503.
  • IHC-P - Anti Hepatitis B Surface Antigen Antibody  ABD12988
    Published customer image:Rabbit anti hepatitis B surface antigen antibody used for the detection of hepatitis S antigen in infected mouse liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Immunohistochemical staining for HBsAg (A-D) and HBcAg (E-H) in liver sections. Treatment with pWPT-HBV-shRNA-1 lentivirus(C, G) or pWPT-HBV-shRNA-2 lentivirus (D, H) reduces the number of HBsAg or HBcAg-positive cells on day 7 respectively compared to the control. The staining was performed on tissue sections from three animals per group. Representative sections are shown (four sections per animals were stained and counted). Numerous HBsAg-positive and HBcAg-positive cells were seen in the empty vector group (A, E) and negative control RNAi group (B, F). No HBsAg-positive stained cells were observed in most fields in pWPT-HBV-shRNA-2 group(D). Some stained cells could be seen in pWPT-HBV-shRNA-1 or shRNA-2 groups(C, G, H) and these were indicated by arrows, but staining were much less intense than that in control groups (100× original magnification, scale bar 200 μm).From: Deng L, Li G, Xi L, Yin A, Gao Y, You W, Wang X, Sun B. Hepatitis B virus inhibition in mice by lentiviral vector mediated short hairpin RNA.BMC Gastroenterol. 2009 Oct 6;9:73.
  • IHC - Anti Hepatitis B Surface Antigen Antibody  ABD12988
    Published customer image:Rabbit anti hepatitis B surface antigen antibody used for the detection of Hepatitis s antigen in guinea pig skin by immunohistochemistry on cryosections.Image caption:Plasmid expression and inflammation in the skin. Guinea pigs were treated with pHBsAg. Expression of plasmid was evaluated at 48 hrs post treatment by IHC (A-IO; B I+EP). Inflammation was measured 96 hrs post treatment and assessed by H&E (C-No treatment; D- IO, E–I+EP) at 100× magnification and 200× magnification (F- I+EP).From: Donate A, Coppola D, Cruz Y, Heller R Evaluation of a Novel Non-Penetrating Electrode for Use in DNA Vaccination.PLoS ONE 6(4): e19181.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Virus
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Additional Information
Immunogen Hepatitis B subtypes ad and ay.
Shelf Life 12 months from date of despatch.
Target/Specificity Rabbit anti hepatitis B surface antigen antibody recognizes Hepatitis B surface antigen, recognizing subtypes ad and ay. Hepatitis B virus is a major causative agent of acute and chronic liver disease in humans.Hepatitis B surface antigen (HBsAg) is a protein component of the viral envelope, which is expressed predominantly in the cytoplasm of infected hepatocytes.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Hepatitis B Surface Antigen Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12988
(40 western blots)
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