|Application ||WB, IHC-F, FC|
|Calculated MW||270835 Da|
|Immunogen||A GST fusion protein using the mouse Notch 1 amino acid sequence 1299-1492.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Neurogenic locus notch homolog protein 1, Notch 1, Motch A, mT14, p300, Notch 1 extracellular truncation, NEXT, Notch 1 intracellular domain, NICD, Notch1, Motch|
|Target/Specificity||ABD13086 specifically recognises Notch 1, one of the four major transmembrane receptors (Notch 1-4) of the Notch signalling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation.Notch 1 is expressed in a range of cells including haematopoietic cells in mouse foetal liver, adult thymus and bone marrow. Notch 1 signalling plays a role in follicular differentiation, tissue homeostasis, and in both CD4+ and CD8+ cell maturation in the thymus. Studies have also implicated Notch 1 in the regulation of lymphopoieisis, myelopoiesis, and neurogenesis.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at -20℃ only (ship +4ºC)|
|Precautions||Anti Notch 1 Antibody, clone 8G10 is for research use only and not for use in diagnostic or therapeutic procedures.|
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