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Anti Human Collagen IV Antibody, clone NLI/53

Mouse Anti-Human Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P02462
Other Accession P29400, P08572, Q01955, Q14031, P53420
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgM
Clone Names NLI/53
Calculated MW 160615 Da
Additional Information
Purification Purified IgM prepared by ion exchange chromatography
Shelf Life 12 months from date of reconstitution.
Gene ID 1282
Other Names Collagen alpha-1(IV) chain, Arresten, COL4A1
Target/Specificity Intended UseTarget SpeciesHumanProduct FormPurified IgM - lyophilisedReconstitutionReconstitute with 1.0 ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AbD Serotec recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.PreparationPurified IgM prepared by ion exchange chromatographyPreservative Stabilisers0.1% Mannitol0.1% DextranPurityApprox. Protein ConcentrationsIgM concentration 0.1 mg/ml after reconstitutionReagents In The KitPreparing The AntibodyTest PrincipleBuffer SolutionPhosphate buffered salineStoragePrior to reconstitution store at +4oC.After reconstitution store at -20oC.Storage in frost-free freezers is not recommended. This product should be stored undiluted.Avoid repeated freezing and thawing as this may denature the antibody.Should this product contain a precipitate we recommend microcentrifugation before use.Shelf Life12 months from date of reconstitution.GO TermsGO:0048407platelet-derived growth factor binding; GO:0001525angiogenesis; GO:0005587collagen type IV; GO:0005605basal lamina; GO:0005201extracellular matrix structural constituent; GO:0030198extracellular matrix organization; GO:0005515protein binding; GO:0032836glomerular basement membrane development; GO:0007411axon guidance; GO:0016525negative regulation of angiogenesis; UniProtP02462; P29400; P08572; Q01955; Q14031; P53420; Entrez GeneCOL4A1; COL4A6; COL4A4; COL4A2; COL4A5; COL4A3; AcknowledgementsRegulatoryFor research purposes only
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human Collagen IV Antibody, clone NLI/53 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD13175
(40 western blots)
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