|Application ||IHC-P, IHC-F, E|
|Other Accession||P29400, P08572, Q01955, Q14031, P53420|
|Calculated MW||160611 Da|
|Purification||Purified IgM prepared by ion exchange chromatography|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Collagen alpha-1(IV) chain, Arresten, COL4A1|
|Target/Specificity||Intended UseTarget SpeciesHumanProduct FormPurified IgM - lyophilisedReconstitutionReconstitute with 1.0 ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AbD Serotec recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.PreparationPurified IgM prepared by ion exchange chromatographyPreservative Stabilisers0.1% Mannitol0.1% DextranPurityApprox. Protein ConcentrationsIgM concentration 0.1 mg/ml after reconstitutionReagents In The KitPreparing The AntibodyTest PrincipleBuffer SolutionPhosphate buffered salineStoragePrior to reconstitution store at +4oC.After reconstitution store at -20oC.Storage in frost-free freezers is not recommended. This product should be stored undiluted.Avoid repeated freezing and thawing as this may denature the antibody.Should this product contain a precipitate we recommend microcentrifugation before use.Shelf Life12 months from date of reconstitution.GO TermsGO:0048407platelet-derived growth factor binding; GO:0001525angiogenesis; GO:0005587collagen type IV; GO:0005605basal lamina; GO:0005201extracellular matrix structural constituent; GO:0030198extracellular matrix organization; GO:0005515protein binding; GO:0032836glomerular basement membrane development; GO:0007411axon guidance; GO:0016525negative regulation of angiogenesis; UniProtP02462; P29400; P08572; Q01955; Q14031; P53420; Entrez GeneCOL4A1; COL4A6; COL4A4; COL4A2; COL4A5; COL4A3; AcknowledgementsRegulatoryFor research purposes only|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Human Collagen IV Antibody, clone NLI/53 is for research use only and not for use in diagnostic or therapeutic procedures.|
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