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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti Mouse Collagen IV Antibody    

Anti Mouse Collagen IV Antibody

Rabbit Anti-Mouse Polyclonal Antibody

     
  • FC - Anti Mouse Collagen IV Antibody  ABD13177
    Published customer image:Retinal gliosis and subretinal neovascularization in RCS rats. Double label immunostaining was performed using antibodies against vimentin and collagen type IV on frozen sections. (A) Non-dystrophic rat at 21 weeks (w) of age showed a normal distribution of retinal vessels confined to the inner retina and absence of glial cells in the subretinal space. (B, C) RCS rats at 6 w (B) and 9 w (C) of age showed activation of Muller cells but without subretinal neovascularization. Muller cell invasion into the subretinal space was more obvious at 9 w compared with 6 w of age (arrows in C). (D) Subretinal neovascularization in RCS rats at 13 w of age. GCL = ganglion cell layer, INL = inner nuclear layer, OPL = outer plexiform layer, ONL = outer nuclear layer, SP = subretinal space, CC = choroidal capillaries. Scale bars: 50 µm.From: Shen W, Chung SH, Irhimeh MR, Li S, Lee S-R, et al. Systemic Administration of Erythropoietin Inhibits Retinopathy in RCS Rats. PLoS ONE 9(8): e104759.
  • WB - Anti Mouse Collagen IV Antibody  ABD13177
    Published customer image:Smooth muscle actin and CollagenIV levels are not altered in Elk3(-/-) knockout retinae. (A) IsolectinB4 and smooth muscle actin (middle and right) staining of retinal flat-mounts of control (upper panel) and Elk3(-/-) knockout animals. (B) IsolectinB4 and collagenIV (middle and right) staining of retinal flat-mounts of control (upper panel) and Elk3(-/-) knockout animals. (C) Quantitation of collagenIV RNA levels in control and Elk3(-/-) knockout retinae (n = 5 independent experiments). (D) Representative pairs of control and Elk3(-/-) knockout retinal lysates tested for smooth muscle actin expression in Western Blot analysis. (E) Quantitation of control and Elk3(-/-) knockout retinal levels of SMA (n = 5 independent experiments). Scale bar in (A, B left and middle) 1000 µm, in (A, B right) 100 µm.From: Weinl C, Wasylyk C, Garcia Garrido M, Sothilingam V, Beck SC, et al. Elk3 Deficiency Causes Transient Impairment in Post-Natal Retinal Vascular Development and Formation of Tortuous Arteries in Adult Murine Retinae. PLoS ONE 9(9): e107048.
  • IF - Anti Mouse Collagen IV Antibody  ABD13177
    Published customer image:Vhltrp1-creKO mice demonstrate a Hif1a-dependent persistence of the pupillary membrane. (A-I) Autofluorescence scanning laser ophthalmoscope imaging (A,D,G), indocyanin-green angiography (ICGA; B,E,H) and collagen IV-stained cryosections (C,F,I) of Vhltrp1-creKO, Vhl;Hif1atrp1-creKO and control littermates at P21 show a persistent pupillary membrane in Vhltrp1-creKO mice that is absent in Vhl;Hif1atrp1-creKO and control mice. CB, ciliary body. Scale bar: 75 µm.From: Lange CA, Luhmann UF, Mowat FM, Georgiadis A, West EL, Abrahams S, Sayed H, Powner MB, Fruttiger M, Smith AJ, Sowden JC, Maxwell PH, Ali RR, Bainbridge JW. Von Hippel-Lindau protein in the RPE is essential for normal ocular growth and vascular development. Development. 2012 Jul;139:2340-50.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IF, E
Primary Accession P02463
Other Accession Q9QZS0, P08122, Q9QZR9
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 160679 Da
Additional Information
Other Species Orangutan,Rat
Purification Purified IgG prepared by antigen column chromatography
Immunogen Collagen IV purified from mouse EHS tumor.
Shelf Life 18 months from date of despatch.
Gene ID 12826
Other Names Collagen alpha-1(IV) chain, Arresten, Col4a1
Target/Specificity Rabbit amti Mouse Collagen IV antibody recognizes mouse collagen type IV. Collagen IV is a 1682 amino acid ~160kDa (predicted) matrx protein and major component of glomerular basement membranes. Multiple isoforms exist each capable of forming triple helical structures with two other chains to form the type IV collagen network. The collagen IV alpha chain can be cleaved between residues 1444-1445 to yield the c-terminal 225 amino acid, ~28kDa arresten fragment, collagen α2(IV) yields a c-terminal canstatin fragment while Collagen α3(IV) yeilds a tumstatin fragment. Collagen IV bears a single collagen IV NC1 (C-terminal non-collagenous) domain (UniProt: Q9QZR9).Mutations in collagen IV genes have been implicated in inherited nephropathies and potentially in cystic kidney disease and intracranial aneurysms (Plaisieret al.2007).Rabbit amti Mouse Collagen IV antibody has been successfully employed for the detection of collagen IV by immunofluorescence and immunohistochemistry in mice (e.g. Tanget al.), rats (Shenet al.2014) and oragutan (Bredieset al.2013).The following cross reactivities have been observed:Mouse type IV100%Mouse types I, II & III
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Mouse Collagen IV Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD13177
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