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Anti GAPDH (N-Terminal) Antibody

Rabbit Anti-Human Polyclonal Antibody

  • WB - Anti GAPDH (N-Terminal) Antibody  ABD13325
    Western blot analysis of whole cell lysate from HeLa, human epithelial adenocarcinoma cells probed with Rabbit anti Human GAPDH at 0.125(A), 0.25(B) and 0.5(C) ug/ml
  • IHC - Anti GAPDH (N-Terminal) Antibody  ABD13325
    Immunohistochemical staining of human liver with Rabbit anti Human GAPDH
  • WB - Anti GAPDH (N-Terminal) Antibody  ABD13325
    HeLa (lane 2) and HepG2 (lane 3) cell lysates were run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lane 1. Rabbit anti Human GAPDH was used as the primary antibody at a dilution of 1/1000. Mouse anti Rabbit Light Chain:HRP MCA6003P was used as the secondary antibody at a dilution of 1/5000 . Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure
  • WB - Anti GAPDH (N-Terminal) Antibody  ABD13325
    Multiplex staining of HeLa whole cell lysate with Mouse anti PCNA detected with Goat anti Mouse IgG (H/L):DyLight®680 shown in red and Rabbit anti GAPDH detected with Sheep anti Rabbit IgG:DyLight®488 shown in green
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04406
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 36053 Da
Additional Information
Other Species M,Rat
Immunogen A peptide corresponding to a 16 amino acid sequence from near the amino-terminus of GAPDH.
Shelf Life 18 months from date of despatch.
Gene ID 2597
Other Names Glyceraldehyde-3-phosphate dehydrogenase, GAPDH,, Peptidyl-cysteine S-nitrosylase GAPDH, 2.6.99.-, GAPDH, GAPD
Target/Specificity Rabbit anti GAPDH (N-Terminal) antibody recognizes human glyceraldehyde 3-phosphate dehydrogenase (GAPDH). GAPDH is a major glycolytic enzyme within the cytosol, which is also involved in a number of intracellular processes including membrane fusion, microtubule bundling, phosphotransferase, DNA replication and DNA repair.GAPDH may be involved in the cellular phenotype of age-related neurodegenerative disorders such as Alzheimer’s and Huntington’s disease.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti GAPDH (N-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD13325
(40 western blots)
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