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Anti Human CD82 (C-Terminal) Antibody

Rabbit Anti-Human Polyclonal Antibody

  • WB - Anti Human CD82 (C-Terminal) Antibody  ABD13331
    Western blot analysis of whole cell lysate from A549 human lung adenocarcinoma cells probed with Rabbit anti Human CD82 at 0.5(A) and 1(B) ug/ml
  • IHC - Anti Human CD82 (C-Terminal) Antibody  ABD13331
    Immunohistological staining of human colon with Rabbit anti Human CD82
  • IF - Anti Human CD82 (C-Terminal) Antibody  ABD13331
    Published customer image:Rabbit anti Human CD82 antibody used for the detection of CD82 clusters on a B cell membrane by immunofluorescence.Image caption:CD53 does not overlap with CD82. A: B cell membrane sheets double stained for CD82 and CD82 were imaged by STED microscopy. Scale bars: 5 μm in whole sheets and 1 μm in zoomed images. Graph: intensity profiles of CD82 (red curves) and CD82 (filled green) as depicted in the corresponding images. B: B cells membrane sheets double stained for CD82 and CD53 were imaged by STED microscopy. Scale bars: 5 μm in whole sheets and 0.5 μm in zoomed images. Graphs: intensity profiles of CD82 (red line) and CD53 (filled green) as depicted in the corresponding images.From: Zuidscherwoude, M. et al. The tetraspanin web revisited by super-resolution microscopy.Sci. Rep. 5, 12201; doi: 10.1038/srep12201 .
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P27701
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 29626 Da
Additional Information
Other Species M,Rat
Immunogen A peptide corresponding to a 15 amino acid sequence close to the carboxy terminus of Human CD82.
Shelf Life 18 months from date of despatch.
Gene ID 3732
Other Names CD82 antigen, C33 antigen, IA4, Inducible membrane protein R2, Metastasis suppressor Kangai-1, Suppressor of tumorigenicity 6 protein, Tetraspanin-27, Tspan-27, CD82, CD82, KAI1, SAR2, ST6, TSPAN27
Target/Specificity Rabbit anti Human CD82 antibody recognises the carboxy-terminal region of human CD82, also known as KAI1, SAR2 and TSPAN27. CD82 is a ~30 kDa multi-pass transmembrane glycoprotein belonging to the tetraspanin (TM4SF) family.CD82 acts as a cancer metastasis suppressor protein (Fenget al.2015), while ubiquitously expressed at moderate to high levels in many normal tissues, its expression is down regulated during tumour progression. Loss of CD82 and p53 is correlated with poor prognosis for patients with prostate and other forms of cancer (Liuet al.2006).CD82 interacts with CD234 (DARC). Tumour cells dislodged from the primary tumour and expressing CD82 interact with CD234 expressed on vascular cells, transmitting a senescent signal to the tumour cells (Tsaiet al2011). Tumour cells that have lost CD82 expression can proliferate and potentially give rise to metastases (Miranti 2009).Two isoforms of CD82 may be generated by alternative splicing. The c-terminal region is not affected and thus Rabbit anti Human CD82 antibody is expected to recognize both isoforms of human CD82 (UniProt: P27701).
Preservative & Stabilisers 0.02% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human CD82 (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD13331
(40 western blots)
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