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>   home   >   Products   >   Primary Antibodies   >   Anti Human Immunodeficiency Virus 1 gp120 Antibody    

Anti Human Immunodeficiency Virus 1 gp120 Antibody

Goat Anti-Viral Polyclonal Antibody

  • WB - Anti Human Immunodeficiency Virus 1 gp120 Antibody  ABD13373
    Published customer image:Goat anti Human Immunodeficiency Virus 1 gp120 antibody used for evaluation of envelope protein fragmants in HeLa cell lysates by western blotting.Image caption:FIP1C depletion and HIV-1 Env incorporation in HeLa cells. (A) The efficiency of depletion of Rab11a-FIPs was measured by real time PCR . Infectivity of released particles following depletion of each FIP family member is shown below. (B) Depletion of FIP1C was confirmed at the protein level by immunoblotting with a FIP1C-specific antiserum. Env levels in both cell lysates and virions harvested from the FIP-depleted HeLa cells were assayed by immunoblotting. (C) Restoration of Env incorporation following shRNA-mediated depletion. Two constructs were used in this experiment: FIP1C cDNA is an shRNA sensitive GFP-tagged plasmid; while FIP1C* includes silent mutations in the shRNA target sequence rendering it shRNA-resistant. 2 ug and 4 ug of each FIP1C construct were used in the repletion assay as indicated at the top of the blot. (D) Infectivity of viral particles from the experiment shown in panel D as evaluated using TZM-bl indicator cells. Lanes are numbered and correspond to the blot above. Statistical comparisons utilized the unpaired t-test, * = p
  • WB - Anti Human Immunodeficiency Virus 1 gp120 Antibody  ABD13373
    Published customer image:Goat anti Human Immunodeficiency Virus 1 gp120 antibody used for evaluation of envelope protein fragmants in HeLa cell lysates by western blotting.Image caption:ab14 enhancement of HIV-1 Env incorporation requires FIP1C and Rab14 depletion does not alter incorporation of MLV or VSV-G Env. A) Titration of Rab14Q70L in HeLa cells with normal FIP1C levels (Scr shRNA lanes) or in cells depleted of FIP1C (FIP1C shRNA lanes). Note that gp120 and gp160 blots were probed with goat anti-gp120/gp160 antisera, while gp41 was probed with murine monoclonal anti-gp41 antibody. B) Hela cells were transfected with NL4-3 or with NL4-3deltaEnv and expression constructs pHCMV-G (for VSV-G) or pCL-Ampho (MLV Env). Depletion of Rab14 was performed using shRNA in the indicated lanes. Cellular and particle Env content was assessed by immunoblotting with specific antisera for HIV gp120/160, VSV-G, or amphotropic MLV Env.From: Qi M, Williams JA, Chu H, Chen X, Wang J-J, Ding L, et al. Rab11-FIP1C and Rab14 Direct Plasma Membrane Sorting and Particle Incorporation of the HIV-1 Envelope Glycoprotein Complex.PLoS Pathog 9(4): e1003278.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q70626
Reactivity Virus
Host Goat
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 96938 Da
Additional Information
Immunogen Recombinant gp160 comprising the C terminus of gp120 and most of gp41.
Shelf Life 18 months from date of despatch.
Other Names Envelope glycoprotein gp160, Env polyprotein, Surface protein gp120, SU, Glycoprotein 120, gp120, Transmembrane protein gp41, TM, Glycoprotein 41, gp41, env
Target/Specificity Goat anti Human Immunodeficiency Virus 1 gp120 antibody recognizes the human immunodeficiency virus envelope protein. The viral envelope protein gp160 is cleaved into fragments gp120 and gp41. gp120 is an exterior envelope glycoprotein on the surface of the virus particle that binds to CD4 and chemokine receptors on macrophages and T-lymphocytes. gp41 is non-covalently bound to gp120 and aids the virus in entering the cell. Goat anti Human Immunodeficiency Virus 1 gp120 antibody reacts with gp120, gp160 and gp41 in western blot assays.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human Immunodeficiency Virus 1 gp120 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD13373
(40 western blots)
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