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>   home   >   Products   >   Primary Antibodies   >   Anti CCT eta Antibody, clone PK/16/8/a    

Anti CCT eta Antibody, clone PK/16/8/a

     
  • WB - Anti CCT eta Antibody, clone PK/16/8/a  ABD13391
    Western blot analysis of HeLa whole cell lysate (1) and HeLa heat stressed whole cell lysate (2) probed with Rat anti CCT eta followed by F(ab')2 Rabbit anti Rat IgG conjugated to HRP and visualized by chemiluminescence
  • WB - Anti CCT eta Antibody, clone PK/16/8/a  ABD13391
    Published customer image:Rat anti CCTη antibody, clone PK/16/8/a used for the detection of CCT&eta in fetal and adult rabbit fibroblasts by western blotting.Image caption:CCT-eta but not CCT-beta protein and mRNA are differentially expressed in fetal versus adult fibroblasts. RNA and protein extracted from fetal and adult fibroblasts were subjected to qRT PCR (a & b) and Western blot (c & d) analyses respectively. CCT-eta mRNA was significantly more abundant in adult fibroblasts when compared to fetal fibroblasts (a); there was no significant difference in CCT-beta message levels between fetal and adult fibroblasts (b). Values are means ± SEM of three independent studies performed in duplicate. Statistical analyses were performed using Student's t test. NS = non-significant. Equal amounts of protein loaded from fetal and adult fibroblasts showed that adult fibroblasts express significantly greater CCT-eta protein(c). In contrast CCT-beta protein levels were not different between fetal and adult fibroblasts (d). Blots shown here are representative of at least three different experiments.From: Satish L, Johnson S, Wang JH-C, Post JC, Ehrlich GD, et al. Chaperonin Containing T-Complex Polypeptide Subunit Eta Is a Specific Regulator of Fibroblast Motility and Contractility. PLoS ONE 5(4): e10063.
  • WB - Anti CCT eta Antibody, clone PK/16/8/a  ABD13391
    Published customer image:Rat anti CCTη antibody, clone PK/16/8/a used for the detection of CCT&eta in fetal and adult rabbit fibroblasts by western blotting.Image caption:siRNAs against CCT-eta and CCT-beta decrease both basal and EGF- induced mRNA and protein levels of their targets in fibroblasts. (a & b) qRT-PCR analysis of CCT-eta and CCT-beta mRNA levels showed effective inhibition of both basal expression and EGF-induction in siRNA-transfected adult fibroblasts. Results are expressed as relative quotient of measured CCT-eta or CCT-beta mRNA and were calculated as a percentage of baseline control levels (100%). Values are means ± SEM of six independent studies, each performed in duplicate. Statistical analyses were performed with Student's t test. Ntx- no transfection; EGF-EGF treatment (1 nM); siRNA-treatment with CCT-eta/CCT-beta siRNA; Scr- treatment with scrambled control siRNA. (c & d) Western blot results using CCT-eta and CCT-beta antibody (1:500) showed effective reduction of CCT-eta and CCT-beta protein levels when siRNA was administered but no decrease when scrambled siRNA was employed. GAPDH was used as a loading control. A representative immunoblot of up to four similar such blots is shown for each analysis.From: Satish L, Johnson S, Wang JH-C, Post JC, Ehrlich GD, et al. Chaperonin Containing T-Complex Polypeptide Subunit Eta Is a Specific Regulator of Fibroblast Motility and Contractility. PLoS ONE 5(4): e10063.
  • WB - Anti CCT eta Antibody, clone PK/16/8/a  ABD13391
    Published customer image:Rat anti CCTη antibody, clone PK/16/8/a used for the detection of CCT&eta in fetal and adult rabbit fibroblasts by western blotting.Image caption:siRNA downregulation of CCT-eta but not CCT-beta reduces a-SMA protein levels in adult fibroblasts. (a & b)Western blot showed effective reduction of both CCT-eta and a-SMA protein levels when CCT-eta siRNA was administered, leaving beta-actin largely unaffected. In contrast, downregulating CCT-beta did not lead to significant reduction in either a-SMA or beta-actin levels. GAPDH was used as loading internal control. A representative immunoblot of up to three similar such blots are shown for each analysis. Ntx- no transfection; EGF-EGF treatment (1 nM); siRNA-treatment with CCT-eta/CCT-beta siRNA; Scr- treatment with scrambled control siRNA.From: Satish L, Johnson S, Wang JH-C, Post JC, Ehrlich GD, et al. Chaperonin Containing T-Complex Polypeptide Subunit Eta Is a Specific Regulator of Fibroblast Motility and Contractility. PLoS ONE 5(4): e10063.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q2NKZ1
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names PK/16/8/a
Calculated MW 59443 Da
Additional Information
Other Species M,H,B,Rb
Purification Purified IgG prepared by affinity chromatography on Protein G
Shelf Life 18 months from date of despatch.
Gene ID 514355
Other Names T-complex protein 1 subunit eta, TCP-1-eta, CCT-eta, CCT7
Target/Specificity Rat anti CCTeta antibody, clone PK/16/8/a recognizes the eta polypeptide of the CCT chaperonin molecule complex.CCTη is a 543 amino acid ~60kDa molecule encoded by the CCT7 gene, a member of the TCP-1 chaperonin family (UniProt: Q2NKZ1)The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.Rat anti CCTeta antibody, clone PK/16/8/a detects a band of ~60kDa in HeLa cell lysates using western blotting under reducing conditions.
Preservative & Stabilisers 0.09%Sodium Azide
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti CCT eta Antibody, clone PK/16/8/a is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD13391
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