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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti Bovine TNF Alpha Antibody, clone CC327    

Anti Bovine TNF Alpha Antibody, clone CC327

Mouse Anti-Bovine Monoclonal Antibody

  • FC - Anti Bovine TNF Alpha Antibody, clone CC327  ABD13409
    Published customer image:Phycoerythrin conjugated Mouse anti Bovine TNF alpha antibody, clone CC327 used for the evaluation of TNF-α expression by bovine PBMC following M. bovisinfection.Image caption:Identification of multifunctional IFN-γ, IL-2 and TNF-α CD4+ cells in natural M. bovis infection. PBMC from naturally M. bovis infected cattle were cultured in the presence of either PPD-B or medium and the co-expression of IFN-γ, IL-2 and TNF-α determined by ICS flow cytometry. Histograms were gated on singlet, live lymphocytes and then all CD4+ cells analysed for all combinations of cytokine productivity. The upper histograms show the total proportion of CD4+ cells staining for expression of IFN-γ, IL-2 or TNF-α following stimulation with PPD-B (left panel) or medium control (right panel). Subgating of the IFN-γ+ and IFN-γ- CD4+ cells provides histograms that represent all possible functionalities for the expressing of the 3 cytokines, as shown in the lower histograms. The numbers indicate percentage of CD4+ cells and data are representative of 1 of 10 naturally infected cattle.From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis.PLoS ONE 6: e29194.
  • E - Anti Bovine TNF Alpha Antibody, clone CC327  ABD13409
    Published customer image:Mouse anti Bovine TNF alpha antibody, clone CC327 used as a capture reagent for the evaluation of TNF production using a sandwich ELISA with biotinylated clone CC328 as a detection reagent.Image caption:Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in bovine experimental S. epidermidis (-?-) and S. simulans (-?-) intramammary infection during the two-week study period (mean ± SEM, n = 8 + 8; TNF-α n = 8 + 7). Milk IL-8 (p = 0.04) and IL-1β (p = 0.03) concentrations were higher in response to infection with S. simulans than with S. epidermidis. The SCC is expressed as a natural logarithm of cells/mL.From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S. Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulansandS. epidermidis.Vet Res. 2011 Mar 17;42:49.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q06599
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names CC327
Calculated MW 25567 Da
Additional Information
Other Species Fallow deer
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Recombinant bovine TNF alpha.
Shelf Life 18 months from date of despatch.
Gene ID 280943
Other Names Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2
Target/Specificity Mouse anti Bovine TNF alpha antibody, clone CC327 recognizes bovine TNF alpha, a 17.5 kDa cytokine, expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes.The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN-beta, NGF, Oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumour and toxic effects and under certain conditions is also capable of self-stimulation and inhibition.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Bovine TNF Alpha Antibody, clone CC327 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD13409
(40 western blots)
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