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Anti DAZL Antibody, clone 3/11A

Mouse Anti-Human Monoclonal Antibody

     
  • IF - Anti DAZL Antibody, clone 3/11A  ABD13410
    Published customer image:Mouse anti Human DAZL antibody, clone 3/11A used for the detection of DAZL expressing cells in ovarian tissue by immunofluorescence.Image caption:Abnormal germ cell proliferation was noted in GR-deficient embryos after sex determination. PGCs were labeled with Dazl , and proliferating cells were labeled with Ki67 . Most of the germ cells were Ki67-positive at E10.5 (A, B) and E11.5 (E, F), but the Ki67 signal was absent in most germ cells in control embryos after sex determination at E13.5 (I, J, white arrow-heads). In Wt1R394W/R394W embryos, germ cells were labeled with Ki67 at E10.5 (C, D) and E11.5 (G, H), similar to control embryos. However, the germ cells in Wt1R394W/R394W embryos remained Ki67-positive (K, L, white arrows) at E13.5. ce, coelomic epithelium; gr, genital ridge; g, gonad. Scale bars: 30 μm. Histogram summarizing selected percentage of gonocytes that were Ki67+ and note that the percentage of Ki67+ gonocytes were significantly increased in Wt1R394W/R394W embryos at E13.5, compared with control embryos at the same stage (M). The mRNA level of CDKI (p15INK4b, p16Ink4A, P21WAF1/Cip1 and p27Kip1) that inhibited the G1/S transition was estimated by real-time RT-PCR at E12.5 and E13.5 and normalized against PGC-specific marker Stella (N, O). * P <0.05, * * P <0.01. Each bar, mean ± SD of n = 3 experiments.From: Chen SR, Zheng QS, Zhang Y, Gao F, Liu YX. Disruption of genital ridge development causes aberrant primordial germ cell proliferation but does not affect their directional migration.BMC Biol. 2013 Mar 5;11:22.
  • WB - Anti DAZL Antibody, clone 3/11A  ABD13410
    Published customer image:Mouse anti Human DAZL antibody, clone 3/11A used for the detection of DAZL in ovarian and testicular tissue lysates by western blotting.Image caption:Western analysis of 1st and 2nd trimester ovaries and testes. In both ovarian (A) and testicular (B) samples VASA (76 kDa) was not detectable in the 1st trimester samples but was present in those from the 2nd trimester. DAZL (33 kDa) was low/undetectable in 1st trimester ovaries (C) whereas it was detectable in ovarian samples from 2nd trimester and testicular samples from both 1st and 2nd trimester (D). OCT4 (42 kDa) was present in both ovaries (E) and testes (F) during both the 1st and 2nd trimester. The loading control in all cases was β-tubulin (51 kDa).From: Anderson RA, Fulton N, Cowan G, Coutts S, Saunders PT. Conserved and divergent patterns of expression of DAZL, VASA and OCT4 in the germ cells of the human fetal ovary and testis.BMC Dev Biol. 2007 Dec 18;7:136.
  • IF - Anti DAZL Antibody, clone 3/11A  ABD13410
    Published customer image:Mouse anti DAZL antibody, clone 3/11A used for the evaluation of DAZL expression on cultured human spermatogonia by immunofluorescenceImage caption:Immunohistochemical analysis of (a) hSSC < 2 weeks.Cells were stained with CD49f, DAZL, VASA, VIMENTIN, and STELLA. For VIMENTIN the cultures were negative. Magnification 20x.From: Sabine Conrad, Hossein Azizi, Maryam Hatami, et al., “Differential Gene Expression Profiling of Enriched Human Spermatogonia after Short- and Long-Term Culture,”BioMed Research International, vol. 2014, Article ID 138350, 17 pages, 2014.
  • IF - Anti DAZL Antibody, clone 3/11A  ABD13410
    Published customer image:Mouse anti Human DAZL antibody, clone 3/11A used for the detection of DAZL expressing cells in ovarian tissue by immunofluorescence.Image caption:The PGCs identity was not changed in Wt1R394W/R394W embryos. PGCs identity was examined by double staining of Stella with PGCs characteristic markers, including Oct4 (A), Dazl (B), SSEA-1 (C), and Blimp1 (D). In Wt1R394W/R394W embryos, PGCs still expressed Stella, SSEA-1, Blimp1, Dazl and pluripotent marker Oct4. Scale bars: 50 μm. PGCs-PGCs interactions, such as E-cadherin and β-catenin were maintained in Wt1R394W/R394W embryos (E, F). Scale bars: 50 μm. The mRNA level of PGCs characteristic markers (Oct4, Dazl, SSEA-1, Blimp1, Nanos2, Figla), and PGCs-PGCs interaction markers (E-cadherin and β-catenin) were detected by real-time RT-PCR, and normalized against PGC-specific marker Stella (G). Each bar, mean ± SD of n = 3 experiments.From: Chen SR, Zheng QS, Zhang Y, Gao F, Liu YX. Disruption of genital ridge development causes aberrant primordial germ cell proliferation but does not affect their directional migration.BMC Biol. 2013 Mar 5;11:22.
  • IHC - Anti DAZL Antibody, clone 3/11A  ABD13410
    Published customer image:Mouse anti Human DAZL antibody used for the detection of DAZL expressing cells in rat testis by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Immunohistochemistry showing localisation of germ cells and 5mC during mid to late gestation. In order to clearly show the localisation of germ cells at all stages of development, we used the specific germ cell cytoplasmic marker DAZL, which survives acid denaturation, in combination with 5mC for immunohistochemistry. The cytoplasm of germ cells is stained for DAZL in blue and 5mC is indicated by brown staining in nuclei. Germ cells (indicated by yellow arrows) are located within seminiferous cords which are surrounded by somatic cells; one seminiferous cord is shown outlined in each image. There are also somatic cells within the seminiferous cords (green arrows). Images show that 5mC is undetectable in germ cells between e14.5 and e18.5 (A to E). 5mC is detectable in germ cells at e19.5 (F, indicated by red arrow) and is more marked in germ cells at e20.5 and e21.5 (G, H). 5mC is present in somatic cells throughout the time course. Scale bar?=?50 μm.From: Rose CM, van den Driesche S, Sharpe RM, Meehan RR, Drake AJ. Dynamic changes in DNA modification states during late gestation male germ line development in the rat.Epigenetics Chromatin. 2014 Aug 7;7:19.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession Q92904
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 3/11A
Calculated MW 33178 Da
Additional Information
Purification Tissue Culture Supernatant.
Immunogen Synthetic peptide corresponding to sequence within the C terminal domain of human DAZL (CRVHHFRRSRAMLKSV).
Shelf Life 18 months from date of despatch.
Gene ID 1618
Other Names Deleted in azoospermia-like, DAZ homolog, DAZ-like autosomal, Deleted in azoospermia-like 1, SPGY-like-autosomal, DAZL, DAZH, DAZL1, DAZLA, SPGYLA
Target/Specificity Mouse anti Human DAZL antibody, clone 3/11A recognizes human Deleted in azoospermia-like, also known as DAZL, DAZ homolog, DAZ-like autosomal, Deleted in azoospermia-like 1 or SPGY-like-autosomal. DAZL is a 295 amino acid ~33 kDa member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is predominantly localized to the cytoplasm.Mutations in this gene have been linked to severe spermatogenic failure and infertility in males.
Preservative & Stabilisers 0.09%Sodium Azide
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti DAZL Antibody, clone 3/11A is for research use only and not for use in diagnostic or therapeutic procedures.
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