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>   home   >   Products   >   Primary Antibodies   >   Anti Bovine CD335 Antibody, clone AKS1    

Anti Bovine CD335 Antibody, clone AKS1

Mouse Anti-Bovine Monoclonal Antibody

     
  • FC - Anti Bovine CD335 Antibody, clone AKS1  ABD13415
    Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD335 followed by Goat anti Mouse IgG (H/L):FITC . A small positive peak representing NK cells can be identified
  • FC - Anti Bovine CD335 Antibody, clone AKS1  ABD13415
    Published customer image:Mouse anti bovine CD335 antibody, clone AKS1 used for the isolation by immunomagnetic separation and evaluation of CD335 expression by flow cytometry of NKp46 expression on bovine peripheral blood mononuclear cells.Image caption:CD2 distribution on bovine NK cells in peripheral blood and during cell culture. (A) CD2 expression by PBMCs from healthy cattle, and in NKp46-selected cells cultured for the indicated time in the presence of 100U/ml rbIL-2. Results were obtained by two-colour flow cytometry, gating for viable lymphocytes. Data shown are from one animal representative of 19. (B) Monitoring of cell divisions of CD2- and CD2+ NK cell populations during three and six days of rbIL-2 (100U/ml) stimulation. NKp46-selected cells were labelled with CFSE the day after isolation and CFSE intensity (filled histograms) measured in flow cytometry on the indicated days, gating for CD2- or CD2+ viable cells. Solid line open histograms show non-dividing NK cells, cultured in the presence of 1U/ml rbIL-2, while broken line open histograms show unlabeled stimulated NK cells. Data shown are from one animal representative of five. (C) Stability of CD2 expression in NK cell subsets cultured separately. NK cells separated with immunomagnetic beads for absence or presence of CD2 were cultured separately for eleven days in the presence of IL-2, monitoring CD2 expression at three different time points (filled histograms). Day 1 corresponds to the day of separation, 24 h after primary NK cell isolation. Open histograms show secondary mAb controls. Data shown are from one animal representative of eight.From: Boysen P, Olsen I, Berg I, Kulberg S, Johansen GM, Storset AK. Bovine CD2-/NKp46+ cells are fully functional natural killer cells with a high activation status. BMC Immunol. 2006 Apr 27;7:10.
  • E - Anti Bovine CD335 Antibody, clone AKS1  ABD13415
    Published customer image:Mouse anti bovine CD335 antibody used for the evaluation of NKp46 expression on bovine peripheral blood mononuclear cells by flow cytometryImage caption:Oenothein B Primes bovine CD335+ cells to respond to IL-18. (A) Bovine PBMCs (105 cells/well) were depleted of CD335+ cells and treated with 20 µg/ml oenothein B or X-VIVO medium alone for 24 hrs. Cells were then washed and treated with 10 ng/ml rhu IL-18 or medium alone for 18 hrs. After incubation, IFN? levels in the supernatant fluids were measured by ELISA. The data are expressed as mean +/- SEM of three independent experiments comparing depleted PBMCs to un-depleted controls tested concurrently. All samples were tested in duplicate or triplicate. Statistical significance was measured by Two-way ANOVA with Bonferroni post-test. *p
  • FC - Anti Bovine CD335 Antibody, clone AKS1  ABD13415
    Published customer image:Mouse anti bovine CD335 antibody used for the evaluation of NKp46 expression on bovine peripheral blood mononuclear cells by flow cytometryImage caption:Oenothein B induces IL-2Ra or CD69 on bovine and human NK cells. (A) Bovine PBMCs (105 cells/well) were treated with 20 µg/ml oenothein B in X-VIVO medium for 24 hrs, and IL-2Ra expression on NK cells was measured by multi-color flow cytometry. Representative examples of two-color flow cytometry plots comparing IL-2Ra staining on oenothein B-treated and untreated bovine NK cells (CD335+) from each animal are shown. (B) Human PBMCs (105 cells/well) were treated with 40 µg/ml oenothein B in cRPMI medium for 48 hrs. CD69 expression on NK cells was then measured by flow cytometry. Representative examples of two-color flow cytometry plots comparing CD69 staining on oenothein B-treated and untreated human NK cells from each donor are shown.From: Ramstead AG, Schepetkin IA, Quinn MT, Jutila MA Oenothein B, a Cyclic Dimeric Ellagitannin Isolated from Epilobium angustifolium, Enhances IFN? Production by Lymphocytes.PLoS ONE 7: e50546.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, FC, Func
Primary Accession Q863H2
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names AKS1
Calculated MW 34494 Da
Additional Information
Other Species American Bison
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Fusion protein consisting of the extracellular region of bovine CD335.
Shelf Life 18 months from the date of despatch
Gene ID 369024
Other Names Natural cytotoxicity triggering receptor 1, Natural killer cell p46-related protein, NK-p46, NKp46, CD335, NCR1
Target/Specificity Mouse anti Bovine CD335 antibody, clone AKS1 recognizes bovine CD335, also known as NKp46 and Natural cytotoxicity triggering receptor 1. CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which functions as an activating receptor. CD335 is expressed by human natural killer cells (Sivoriet al.1997). The bovine homologue is expressed on bovine NK cells (Storsetet al.2004) and no expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes.Clone AKS1 is reported to activate lysis of FcgammaR-expressing cell line P815, by IL-2 activated NKp46+ cells (Storsetet al.2004).
Preservative & Stabilisers None present
Storage Store at -20℃ only.
PrecautionsAnti Bovine CD335 Antibody, clone AKS1 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD13415
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