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>   home   >   Products   >   Primary Antibodies   >   Anti Mouse MHC Class I H-2b/D/P/Q/w16 Antibody, clone ER-HR52    

Anti Mouse MHC Class I H-2b/D/P/Q/w16 Antibody, clone ER-HR52

Rat Anti-Mouse Monoclonal Antibody

  • WB - Anti Mouse MHC Class I H-2b/D/P/Q/w16 Antibody, clone ER-HR52  ABD13418
    Published customer image:Rat anti Mouse MHC Class I H-2b/D/P/Q/w16 antibody, clone ER-HR52 used for the identification of MHC class I expressing cells in mouse brain by immunofluorescence.Image caption:Dynamic expression of H-2Kb/Db in mouse hippocampus.(A) At P15, MHCI protein signals were overlapped with those of NeuN (red, a marker for neurons) but not with GFAP (red, a marker for astrocytes). Scale bar: 50 µm. (B) The mRNA of H-2Kb and H-2Db was dynamically expressed in mouse hippocampus. Data was shown as ratio compared with P4 and was calculated from three independent experiments. (C) Western blot analysis depicting the expression of MHC-I protein during the development stages of hippocampus. (D) Expression of MHC-I protein was quantified as the ratio of band density to that of Tuj1. Data was presented as ratio compared with P4 and was calculated from three independent experiments.From: Lv D, Shen Y, Peng Y, Liu J, Miao F, Zhang J Neuronal MHC Class I Expression Is Regulated by Activity Driven Calcium Signaling.PLoS ONE 10(8): e0135223.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names ER-HR52
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Adherent FI (CBAXBL) bone marrow stromal cells
Shelf Life 18 months from date of despatch.
Target/Specificity Rat anti Mouse MHC Class I H-2b/D/P/Q/w16 antibody, clone ER-HR52 recognizes a polymorphic epitope present on murine MHC class I molecules, which are expressed at varying levels on the majority of nucleated cells. Clone ER-HR52 specifically recognizes mouse strains with the haplotypes H-2b, w16 and H-2d, p, q. Mouse strains with the haplotypes H-2f, r, s, w17, w23, w27 show weak reactivity with this antibody.The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Mouse MHC Class I H-2b/D/P/Q/w16 Antibody, clone ER-HR52 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD13418
(40 western blots)
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