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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti Human CD19 Antibody, clone LE-CD19    

Anti Human CD19 Antibody, clone LE-CD19

Mouse Anti-Human Monoclonal Antibody

  • IHC-P - Anti Human CD19 Antibody, clone LE-CD19  ABD13426
    Staining formalin-fixed, paraffin-embedded human tonsil with Mouse anti Human CD19
  • WB - Anti Human CD19 Antibody, clone LE-CD19  ABD13426
    Western blot analysis of Raji human Lymphoblastic Burkitt's lymphoma whole cell lysate probed with Mouse anti Human CD19 antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15391
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names LE-CD19
Calculated MW 61128 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen CD19 peptide CGPDPAWGGGGRMGTWSTR (C-terminus) coupled to KLH.
Shelf Life 18 months from date of despatch.
Gene ID 930
Other Names B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, CD19, CD19
Target/Specificity Mouse anti Human CD19 antibody, clone LE-CD19 recognizes an epitope within the C-terminal cytoplasmic tail sequence of human CD19, a single pass type I transmembrane glycoprotein containing two C2 type Ig-like domains in the N-terminal extracellular region and four potential phosphorylation sites for tyrosine together with a single serine in the cytoplasmic region.Human CD19 is expressed on virtually all cells of the B-cell lineage with the exception of plasma cells and plays a regulatory role in B-cell differentiation and prloliferation.B-cells are essential for antibody production and mutations in the CD19 gene can lead to an immunodeficiency syndrome, CIVD3 characterized by hypogammaglobulinemia leading to recurrent infections and the inability to mount an antibody mediated response to immune insult. Although immunoglobulin production is impaired B-cell precursors appear in normal numbers together with some reduction in more mature B-cell forms (van Zelm et al.2006). B-cells have also been implicated in the progression and pathogenesis of multiple sclerosis and are common components of both active and chronic MS lesions and well as the CSF (Ritchieet al.2004)Mouse anti Human CD19 antibody, clone LE-CD19 has been successfully employed for the immunohistochemical demonstration of CD19 in formalin fixed, paraffin embedded tissues (Streeck, H. et al.2011) and for the detection of CD19 in cell lysates by Western blotting.
Preservative & Stabilisers 0.09%Sodium Azide
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human CD19 Antibody, clone LE-CD19 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD13426
(40 western blots)
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