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Anti ALIX Antibody, clone 3A9

  • WB - Anti ALIX Antibody, clone 3A9  ABD13432
    Western blot analysis of HeLa whole cell lysate probed with MOUSE ANTI ALIX F(ab')2 Rabbit anti Mouse IgG:HRP
  • WB - Anti ALIX Antibody, clone 3A9  ABD13432
    Published customer image:Mouse anti Alix antibody, clone 3A9 used for the evaluation of ALIX expression in tumors by western blotting.Image caption:Lipid analysis of mesothelin. (A) Tandem mass spectrometry (ESI_MS2) of choline-containing phospholipids, phosphatidylcholine and sphingomyelin . Lipids were extracted by the chloroform/methanol solvent Folch mixture from their association with the immunoprecipitated mesothelin from OVCAR3 cell culture supernatant. The high proportion of SM relatively to PC in the supernatant is revealed by the amplitude of peak m/z 703 compared with peaks at m/z 732 (32:1 PC), 760 (34:1 PC) and 786 (36:2 PC). Parent phospholipids of the phosphorylcholine ion (+184) are indicated as (total carbon number in acyl chains:double-bond number PC). Cholesterol assay by MS2 is obtained after acetylation of the non esterified sterol by recording of the transition 446 (acetyl-cholesterol + NH+4) to 369 (not shown). SM/cholesterol molar ratios are given as mole/mole after calibration. (B) Western Blot analysis of immunoprecipitated mesothelin from tumor conditioned media (right lane) and tumor cell lysates (left lane) as positive control. Membranes were probed with anti-Mesothelin, -TSG101, - Alix, or β actin, as indicated. Signals were detected by ECL.From: Dangaj D, Abbott KL, Mookerjee A, Zhao A, Kirby PS, Sandaltzopoulos R, et al. Mannose Receptor Engagement by Mesothelin GPI Anchor Polarizes Tumor-Associated Macrophages and Is Blocked by Anti-MR Human Recombinant Antibody.PLoS ONE 6: e28386.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8WUM4
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 3A9
Calculated MW 96023 Da
Additional Information
Other Species H,X,Rat,M
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Alix fusion protein.
Shelf Life 18 months from date of despatch.
Gene ID 10015
Other Names Programmed cell death 6-interacting protein, PDCD6-interacting protein, ALG-2-interacting protein 1, ALG-2-interacting protein X, Hp95, PDCD6IP, AIP1, ALIX, KIAA1375
Target/Specificity Mouse anti ALIX antibody, clone 3A9 recognizes the apoptosis-linked gene-2 interacting protein X (ALIX), also known as Programmed cell death 6-interacting protein or Hp95. ALIX is a 868 amino acid ~90kDa cytoplasmic protein containing a single BRO1 domain implicated in endosomal targeting (Kimet al.2005).ALIX was first reported to play a role in apoptosis dependent on its association with PDCD6(Mahul-Mellieret al.2006) but has subsequently shown to be involved in endocytosis (Matsuoet al.2004), viral budding (Irieet al.2008) and actin cytoskeletal assembly (Panet al.2006).Mouse anti Alix, clone 3A9 has been used extensively for the detection of ALIX by Western blotting and for the identification of an ALIX homologue in the sea urchin Paracentrotus lividus(Romancinoet al.2013).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti ALIX Antibody, clone 3A9 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD13432
(40 western blots)
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