|Application ||IHC-F, IF, FC|
|Purification||Purified IgM prepared by ammonium sulphate precipitation followed by gel filtration|
|Immunogen||THP1 (human acute monocytic leukaemia cells)|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti Human CD65s antibody, clone VIM-2 selectively recognizes the sialylated form of human CD65, known as CD65s (VIM-2 antigen), a leucocyte carbohydrate antigen expressed by granulocytes, monocytes and leukaemic cells of myelomonocytic lineage.CD65s is aberrantly expressed on some acute myeloid leukaemias (AML) and clone VIM-2 has been reliably used as a marker for distinguishing between mature and undifferentiated AML. During normal myelopoiesis, expression of CD65s follows the disappearance of the progenitor antigen CD34.Cross-linking of the CD65s antigen using clone VIM-2, has been shown to induce phagocyte cytoplasmic calcium flux, oxidative burst and degranulation (Lund-Johansenet al.1992).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Human CD65s Antibody, clone VIM-2 is for research use only and not for use in diagnostic or therapeutic procedures.|
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