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Anti Human CD65s Antibody, clone VIM-2

Mouse Anti-Human Monoclonal Antibody

     
  • FC - Anti Human CD65s Antibody, clone VIM-2  ABD13438
    Staining of human peripheral blood granulocytes with Mouse anti Human CD65s:Low Endotoxin
  • FC - Anti Human CD65s Antibody, clone VIM-2  ABD13438
    Staining of human peripheral blood granulocytes with Mouse anti Human CD65s:Alexa Fluor® 647
  • FC - Anti Human CD65s Antibody, clone VIM-2  ABD13438
    Staining of human peripheral blood granulocytes with Mouse anti Human CD65s:FITC
  • FC - Anti Human CD65s Antibody, clone VIM-2  ABD13438
    Staining of human peripheral blood granulocytes with Mouse anti Human CD65s:RPE
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-F, IF, FC
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgM
Clone Names VIM-2
Additional Information
Purification Purified IgM prepared by ammonium sulphate precipitation followed by gel filtration
Immunogen THP1 (human acute monocytic leukaemia cells)
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti Human CD65s antibody, clone VIM-2 selectively recognizes the sialylated form of human CD65, known as CD65s (VIM-2 antigen), a leucocyte carbohydrate antigen expressed by granulocytes, monocytes and leukaemic cells of myelomonocytic lineage.CD65s is aberrantly expressed on some acute myeloid leukaemias (AML) and clone VIM-2 has been reliably used as a marker for distinguishing between mature and undifferentiated AML. During normal myelopoiesis, expression of CD65s follows the disappearance of the progenitor antigen CD34.Cross-linking of the CD65s antigen using clone VIM-2, has been shown to induce phagocyte cytoplasmic calcium flux, oxidative burst and degranulation (Lund-Johansenet al.1992).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human CD65s Antibody, clone VIM-2 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD13438
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