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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   Anti Human Protein Gene Product 9.5 Antibody, clone 13C4    

Anti Human Protein Gene Product 9.5 Antibody, clone 13C4

Mouse Anti-Human Monoclonal Antibody

  • WB - Anti Human Protein Gene Product 9.5 Antibody, clone 13C4  ABD13463
    Rat brain probed with Mouse anti Human Protein Gene Product 9.5
  • IF - Anti Human Protein Gene Product 9.5 Antibody, clone 13C4  ABD13463
    Published customer image:Mouse anti Human protein gene product 9.5 antibody, clone 13C4 used for the detection of PGP9.5 immunoreactive nerve fibers in porcine gastric mucosa by immunofluorescence.Image caption:Tissue sampling and cell measurements. (a) Picture presenting the interior surface of the"experimental animal"stomach, exposed by cutting along the greater curvature of the stomach. Ulcers are indicated by red arrows. (b) Microphotograph showing Haematoxylin and Eosin staining of the gastric ulcer margin. The gastric mucosa shows a defect (red ellipse with the inscription “Ulcer” and arrows) that extends into the deep muscular layers. (c) Diagram showing the method of tissue sampling. The tracer injection site is indicated by the violet arrows. The red vertical stripes symbolize the subsequent 0.5 cm thick transverse sections taken in specific intervals (section I—1.5 cm; section II—3.5cm; section III—5.5 cm; section IV—7.5cm) starting from the pyloric sphincter (section 0—blue vertical stripe). Subsequently, each of these sections (I, II, III, IV) was cut into 20 µm thick cryostat consecutive microscopic slices for future processing. The red circle symbolizes the localization of ulcer induced in experimental animals. (d, e, f) Photomicrographs showing: (d) bundles of PGP-immunoreactive fibers and perikarya . Double arrow points to the perikaryon which was fast blue positive [the perimeter (l) and area (A) of the cell body are included]; (e) fast blue positive cell body (double arrow) with lines indicating its dimensions; (f) the image formed by merging both channels (green: PGP 9.5 and blue: FB).From: Zalecki M The Influence of Antral Ulcers on Intramural Gastric Nerve Projections Supplying the Pyloric Sphincter in the Pig (Sus scrofa domestica)—Neuronal Tracing Studies.PLoS ONE 10(5): e0126958.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09936
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names 13C4
Calculated MW 24824 Da
Additional Information
Other Species Rat,Gp,Pig
Purification Purified IgG prepared by affinity chromatography on Protein A
Immunogen Native, from brain
Shelf Life 18 months from date of despatch.
Gene ID 7345
Other Names Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1,, 6.-.-.-, Neuron cytoplasmic protein 9.5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, UCHL1
Target/Specificity Mouse anti Human protein gene product 9.5 antibody, clone 13C4 recognizes protein gene product 9.5 (PGP9.5), a ubiquitin hydrolase which is widely expressed in neuronal tissues and represents 1-2% of total soluble brain proteins. PGP9.5, also known as ubiquitin C-terminal hydrolase 1 (UCHL-1), is involved in the regulation of the ubuiquitin pathway.This product stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibres in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumours of the DNES. Also stains neuroendocrine cells in human adult gut (unlike 31A3).Clones 31A3 and 13C4 each recognise a different epitope towards the N-terminus of the protein.
Preservative & Stabilisers 0.09%Sodium Azide
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human Protein Gene Product 9.5 Antibody, clone 13C4 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD13463
(40 western blots)
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