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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   Anti Human Substance P Antibody    

Anti Human Substance P Antibody

Rabbit Anti-Human Polyclonal Antibody

     
  • IHC-P - Anti Human Substance P Antibody  ABD13470
    Staining of paraffin embedded human appendix with Rabbit anti Human Substance P
  • IF - Anti Human Substance P Antibody  ABD13470
    Published customer image:Rabbit anti Human Substance P antibody used to demonstrate substance P staining in keratocytes by immunofluorescence.Image caption:Substance P and neurokinin A expression in keratocytes of cornea tissue sections and in cultured primary keratocytes. (A-B) Substance P (A) and neurokinin A (B) EIA of central and peripheral cultured keratocytes. 250,000 cells were grown for 24h in 6 well plates in DMEM/F12 supplemented with either 2% or 0% FBS. Supernatant was collected, cells were lysed and subjected to EIA. (A) SP levels were significantly higher in supernatants of central keratocytes grown in medium supplemented with 2% FBS than in peripheral keratocytes grown in medium supplemented with 2% FBS (***p<0.001). SP secretion was significantly higher in central keratocytes grown in 2% FBS than central keratocytes grown in 0% FBS (***p<0.001). There were no significant differences in SP levels in lysates of keratocytes. (B) NKA secretion was significantly higher in central keratocytes grown in 2% FBS than in central keratocytes grown in 0% FBS (*p<0.05). (C) Keratocytes in sections (left panel), as well as cultured central (middle panel) and peripheral (right panel) keratocytes, were labeled with an antibody targeting SP (top row; TRITC; red) and double stained with DAPI to visualize the nuclei . Sections and cultured cells were also labeled with an antibody targeting full-length NK-1R (bottom row; Alexa Fluor 488; green) and double stained with DAPI. Both the keratocytes in tissue sections and the cultured keratocytes expressed SP and a full-length version of NK-1R. (D) Keratocytes in sections (left panel), as well as cultured central (middle panel) and peripheral (right panel) keratocytes, were labeled with an antibody targeting NKA (top row; TRITC; red) and NK-2R (bottom row; TRITC; red) and double stained with DAPI. Both the keratocytes in tissue sections and the cultured keratocytes expressed NKA and NK-2R. (E-F) 250,000 central keratocytes or peripheral keratocytes grown in DMEM/F12 supplemented with either 2% or 0% FBS were subjected to western blot analysis for NK-1R (46 kDa) and NK-2R (48 kDa) expression. (E) Expression of NK-1R was significantly higher in central keratocytes grown in 2% FBS than in peripheral keratocytes grown in same conditions (***p<0.001). Expression of NK-1R was also significantly higher in central keratocytes grown in 2% FBS than in central keratocytes grown in 0% FBS (***p<0.001). (F) Expression of NK-2R was significantly higher in central keratocytes grown in 0% FBS than in peripheral keratocytes grown in same conditions (***p<0.001). NK-2R expression was significantly higher in central keratocytes grown in 0% FBS than in central keratocytes grown in 2% (***p<0.001). n.s. (non significant; p>0.05). β-actin (42 kDa) served as a loading control. Values are means ± SD.From: Sloniecka M, Le Roux S, Boman P, Byström B, Zhou Q, Danielson P Expression Profiles of Neuropeptides, Neurotransmitters, and Their Receptors in Human Keratocytes In VitroandIn Situ.PLoS ONE 10(7): e0134157.
  • IF - Anti Human Substance P Antibody  ABD13470
    Published customer image:Rabbit anti Human Substance P antibody used for the detection of substance P in human Achilles tendon cells by immunofluorescence.Image caption:Human Achilles tendon cells in culture immunopositive for SP and vimentin. (a) Cells stained with DAPI , to mark nuclei, and immunolabeled for vimentin (red; TRITC-staining). (b) Same cells stained with DAPI and immunolabeled for SP (green; FITC-staining). Clear SP-reactions are seen close to the nuclei . (c) Digitally merged image of a and b; SP appearing in yellow colour.From: Backman LJ, Fong G, Andersson G, Scott A, Danielson P Substance P Is a Mechanoresponsive, Autocrine Regulator of Human Tenocyte Proliferation.PLoS ONE 6: e27209.
  • IF - Anti Human Substance P Antibody  ABD13470
    Published customer image:Rabbit anti Human Substance P antibody used for the detection of substance P in human Achilles tendon cells by immunofluorescence.Image caption:Human Achilles tendon cells in culture immunopositive for SP and vimentin. (a) Cells stained with DAPI , to mark nuclei, and immunolabeled for vimentin (red; TRITC-staining). (b) Same cells stained with DAPI and immunolabeled for SP (green; FITC-staining). Clear SP-reactions are seen close to the nuclei . (c) Digitally merged image of a and b; SP appearing in yellow colour.From: Backman LJ, Fong G, Andersson G, Scott A, Danielson P Substance P Is a Mechanoresponsive, Autocrine Regulator of Human Tenocyte Proliferation.PLoS ONE 6: e27209.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, IF
Primary Accession P20366
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 15003 Da
Additional Information
Other Species C,M
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Synthetic substance P conjugated to Keyhole limpet hemocyanin.
Shelf Life 18 months from date of despatch.
Gene ID 6863
Other Names Protachykinin-1, PPT, Substance P, Neurokinin A, NKA, Neuromedin L, Substance K, Neuropeptide K, NPK, Neuropeptide gamma, C-terminal-flanking peptide, TAC1, NKA, NKNA, TAC2
Target/Specificity Rabbit anti Human Substance P antibody recognizes human Protachykinin-1, also known as PPT. Protachykinin-1 is a 129 amino acid ~15kDa tachychinin involved in the excitation of neurons. Protachykinin-1 can be cleaved into a number of chains including substance P, neurokinin A, neuropeptide K, neuropeptide γ and c-terminal flankning peptide. Rabbit anti Human Substance P antibody recognizes an epitope withing the substance P region of the molecule. There are potentially four isoforms of Protachykinin-1 (α - δ) formed by alternative splicing, all containing the substance P peptide region (UniProt: P20366).Rabbit anti Human Substance P antibody stains substance P secreting cells of the external median eminance of the hypothalamus and specific cells of the GI tract. Rabbit anti Human Substance P antibody also stains neuronal structures.
Preservative & Stabilisers 0.09%Sodium Azide
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human Substance P Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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