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Anti Substance P Antibody, clone NC1/34

     
  • IF - Anti Substance P Antibody, clone NC1/34  ABD13471
    Published customer image:Tachykinin-like immunoreactivity in nerve fascicles. Nerve fascicles and parts of nerve fascicles (N) of sections from gastrocnemius (A-C, E, F) and soleus (D) muscle specimens are shown. The sections were processed for demonstration of tachykinin-like immunoreaction (A-E). In (F) the reaction pattern after preabsorption with SP peptide is shown (the region in F corresponds to the region in E). The antibodies applied were sc-14104 (A-C, E) and 8450–0505 (D). The specimens were from the control group (A, B, E, F) and from the 6w group, non-exercised side, focal myositis area (C, D). There are immunoreactivities in nerve fascicles in the form of punctuate reactions (arrows A, C-E). These are grouped, as especially seen in (C), but also to some extent in (D). There are no reactions in the nerve fascicle in (B), which is built up of myelinated nerve fibers, and in the control section in (F). MF refers to muscle fibers. Magnification x200 (B, C), x315 (A, D, E, F).From: Song Y, Stål PS, Yu J-G, Forsgren S. Bilateral increase in expression and concentration of tachykinin in a unilateral rabbit muscle overuse model that leads to myositis. BMC Musculoskelet Disord [Internet]. Springer Science + Business Media; 2013;14(1):134.
  • FC - Anti Substance P Antibody, clone NC1/34  ABD13471
    Published customer image:A,B. Tachykinin-like immunoreactivity in cells in inflammatory infiltrate of myositis area. Sections of a specimen from a soleus muscle specimen of the 6w group, non-exercised side. In (A), the section had been immunostained with 8450–0505, and in (B), the section was immunostained with 8450–0505 after preabsorption with tachykinin (larger area is covered in B than in A). Immunoreactivity is seen in cells in (A), but no reaction is visible in (B). Arrows point at cells of inflammatory infiltrate. The immunoreactions show partly a granular appearance (A). Magnification x315. c,d. Immunostaining with polyclonal and monoclonal tachykinin antibodies. A part of a focal myositis area of a section of a gastrocnemius muscle specimen from the 1w group, exercised side, double-stained for tachykinin using the sc-14104 (C) and 8450–0505 (D) antibodies, shown at low magnification. Note that the cells of an inflammatory infiltrate are immunolabelled in both (C) and (D) , whereas the wall of a small vessel is only immunostained in (C) . Magnification x315.From: Song Y, Stål PS, Yu J-G, Forsgren S. Bilateral increase in expression and concentration of tachykinin in a unilateral rabbit muscle overuse model that leads to myositis. BMC Musculoskelet Disord [Internet]. Springer Science + Business Media; 2013;14(1):134.
  • IF - Anti Substance P Antibody, clone NC1/34  ABD13471
    Published customer image:Rat anti substance P antibody, clone NC1/34 used for the detection of substance P expressing cells in tendon tissue.Image caption:Double stainings for PARs and substance P. A-D shows PARs [1]-[4] and E-H shows corresponding SP-staining; PAR-1 (A) is co-localised with SP-positive nerve fibres (E) . The same was seen for PAR-2 (nerve fascicle in B&F), PAR-3 (nerve fascicle in C&G) and PAR-4 (D&H, arrows show perivascular nerve fibres).From: Christensen, J. Alfredson, H. and Andersson, G."Protease-activated receptors in the Achilles tendon–a potential explanation for the excessive pain signalling in tendinopathy".Mol Pain 11: 13. 2015.
  • IF - Anti Substance P Antibody, clone NC1/34  ABD13471
    Published customer image:Rat antiSubstance P antibody, clone NC1/34 used for the identification of substance P immunoreactive neurons in porcine dorsal root ganglia by immunofluorescence.Image caption:Representative images of substance P-positive (SP+) dorsal root ganglia -urinary bladder-projecting neurons in BTX-treated animals. All images were taken separately from blue (a,d,g,j,m,p), green (b,e,h,k,n,r) and red (c,f,i,l,o,s) fluorescent channels; a–c one fast blue-positive (FB+) neuron (a, blue, arrow), which simultaneously contains SP (b, green, arrow) and pituitary adenylate cyclase activating peptide-PACAP (c, red, arrow). d–f One FB+ neuron (d, blue, arrow), which is simultaneously SP+ (e, green, arrow) and calcitonin gene-related peptide-positive (CGRP+, f, red, arrow). g–i One FB+ neuron (g, blue, arrow), which is simultaneously SP+ (h, green, arrow) and galanin-positive (GAL+, i, red, arrow). j–l One FB+ neuron (j, blue, arrow), which simultaneously contains neuronal nitric oxide synthase-nNOS (k, green, arrow) and SP (l, red, arrow). m–o One FB+ neuron (m, blue, arrow), which is simultaneously SP+ (n, green, arrow) and calbindin-negative (CB-, o, red, arrow). p-s One FB+ neuron (p, blue, arrow), which simultaneously contains SP (r, green, arrow) and somatostatin-SOM (s, red, arrow). Bars 50 μm (a–s).From: Bossowska A, Lepiarczyk E, Mazur U, Janikiewicz P, Markiewicz W. Botulinum Toxin Type A Induces Changes in the Chemical Coding of Substance P-ImmunoreactiveDorsal Root Ganglia Sensory Neurons Supplying the Porcine Urinary Bladder.Toxins . 2015 Nov 16;7:4797-4816.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, IF
Primary Accession P20366
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names NC1/34
Calculated MW 15003 Da
Additional Information
Other Species Mammalian species,Pig,Pigeon,Bichir,Rb,H,C,Chinchilla,Camel,Amphibia,Cockroach,Sh,Cowbird,Rat,Spadefoot toad,Homarus sp.,Mudpuppy,Lamprey,CrayF,Zf,Bullfrog,B,Gp,Calanus sp.,Crustacea,Cancer sp.,Panilurus sp.
Immunogen Substance P conjugated to Bovine Serum Albumin.
Shelf Life 18 months from date of despatch.
Gene ID 6863
Other Names Protachykinin-1, PPT, Substance P, Neurokinin A, NKA, Neuromedin L, Substance K, Neuropeptide K, NPK, Neuropeptide gamma, C-terminal-flanking peptide, TAC1, NKA, NKNA, TAC2
Target/Specificity Rat anti substance P antibody, clone NC1/34 recognizes the COOH terminal end of substance P, a short polypeptide neurotransmitter that regulates the excitability of dorsal horn nociceptive neurons. It is known to have a role in several physiologic activities such as pain transmission, the vomiting reflex, salivary secretion and smooth muscle contraction.5% reactivity is observed with eledoisin. It does not react with Leu or Met-enkephalin, somatostatin or beta-endorphin.
Preservative & Stabilisers 0.05% Thiomersal
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Substance P Antibody, clone NC1/34 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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