|Application ||WB, E|
|Calculated MW||16318 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||CHO expressed recombinant ovine GM-CSF.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, CSF, CSF2|
|Target/Specificity||Mouse anti Sheep GM-CSF antibody, clone 8D8 recognizes both recombinant and native ovine granulocyte / macrophage colony-stimulating factor (GM-CSF), also known as Colony stimulating factor (CSF). GM-CSF is one of several cytokines that regulate the survival, growth and differentiation of haemopoietic cells. GM-CSF is secreted from a range of cells including macrophages, T-cells, endothelial cells and fibroblasts in response to antigen, LPS or mitogen stimulation. Mouse anti Sheep GM-CSF antibody, clone 8D8 may be used as a capture antibody in ELISA assays with biotinylated Mouse anti Sheep GM-CSF antibody, (clone 3C2 ) as a detection reagent for the detection of ovine GM-CSF. Clone 8D8 has been reported to neutralize the activity of both recombinant and native ovine GM-CSF (Entricanet al.1996). Removal of the preservative is recommended prior to use in functional assays - AbD Serotec recommend the use of a Slide-A-Lyser® dialysis cassette for this purpose.|
|Preservative & Stabilisers||0.09%Sodium Azide|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Sheep GM-CSF Antibody, clone 8D8 is for research use only and not for use in diagnostic or therapeutic procedures.|
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