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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti Mouse CD44v6 Antibody, clone 9A4    

Anti Mouse CD44v6 Antibody, clone 9A4

Rat Anti-Mouse Monoclonal Antibody

  • IF - Anti Mouse CD44v6 Antibody, clone 9A4  ABD13510
    Published customer image:Rat anti Mouse CD44v6 antibody used for the identification of CD44v6 expressing luminal progenitor cells by immunofluorescence.Image caption:Molecular and phenotypic characteristics of luminal progenitors isolated from virgin and early pregnant females. (A) q-PCR analysis of gene expression levels in mammary Lu-neg and Lu-pos cells isolated from early pregnant females (P-8d). Mean ratios of values normalized to Gapdh expression are shown. Lu-neg/Lu-pos and Lu-pos/Lu-neg ratios are presented in upper and lower panels, respectively. Data are from two independent cell preparations. (B) Icam-1, Met, and Hgf expression in Lu-neg, Lu-pos, and basal/myoepithelial cells, as determined by q-PCR. Cells were isolated from mammary glands of 8-day-pregnant mice. The q-PCR values were normalized to Gapdh expression and represent mean from two independent preparations. (C) Immunodetection of CD44v6 and K8 in cytocentrifuged Lu-neg and Lu-pos cells freshly isolated from mammary glands of mature virgin mice. In upper panels, nuclei were stained with DAPI. Bar, 15 μm.From: Di-Cicco A, Petit V, Chiche A, Bresson L, Romagnoli M, Orian-Rousseau V, Vivanco Md, Medina D, Faraldo MM, Glukhova MA, Deugnier MA. Paracrine Met signaling triggers epithelial-mesenchymal transition in mammary luminal progenitors, affecting their fate.Elife. 2015 Jul 13;4.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15379
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG1
Clone Names 9A4
Calculated MW 85617 Da
Additional Information
Immunogen GST-CD44v6 fusion protein.
Shelf Life 18 months from date of despatch.
Gene ID 12505
Other Names CD44 antigen, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Hermes antigen, Hyaluronate receptor, Lymphocyte antigen 24, Ly-24, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I, CD44, Cd44, Ly-24
Target/Specificity Rat anti Mouse CD44v6, clone 9A4 recognizes an epitope encoded by exon v6 on the variant portion of murine CD44. CD44v6 has been identified along with ICAM-1 as a co-receptor for Met and in mammary epithelia appears to act at the primary Met co-receptor (Di-Ciccoet al2015).Rat anti Mouse CD44v6, clone 9A4 has been used successfully for the identification of CD44v6 expressing cells by immunofluorescence (Di-Ciccoet al.2015), western blotting (Loet al.2012). immunohistochemistry (Weilengaet al.1999) along with ELISA and flow cytometry (Weisset al.1997).
Preservative & Stabilisers 0.09%Sodium Azide
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Mouse CD44v6 Antibody, clone 9A4 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD13510
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