Anti Human Troponin I (Cardiac/Dephosphorylated) Antibody, clone 22B11
Mouse Anti-Human Monoclonal Antibody
|Application ||WB, E|
|Calculated MW||24008 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Dephosphorylated form of human cardiac Troponin I.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Troponin I, cardiac muscle, Cardiac troponin I, TNNI3, TNNC1|
|Target/Specificity||Mouse anti Human Troponin I (Cardiac/Dephosphorylated) antibody, clone 22B11 recognizes the dephosphorylated form of human cardiac Troponin I (cTnI). Troponin I is a 24kDa subunit of Troponin, a protein found on muscle tissue responsible for muscle contraction. When calcium binds to Troponin C, a conformational change moves Troponin I and tropomyosin out of the way of the myosin binding sites on the actin filaments in muscles. This allows cross-bridging to occur and the muscle to contract. Levels of cTnI in serum are commonly used as a marker for cardiac cell damage and death. Phosphorylation of cTnI results in changes in the activity and function of Troponin. One possible form of phosphorylation is by Protein kinase A. Protein kinase A phosphorylation of serine 22 and serine 23 reduces calcium sensitivity of Troponin and leads to an acceleration in relaxation. Changes in cTnI phosphorylation have been reported in failing hearts. Mouse anti Human Troponin I (Cardiac/Dephosphorylated) antibody, clone 22B11 recognizes the dephosphorylated form of cTnI. It specifically recognizes amino acid residues 20-24 on cTnI. The antibody will recognize free dephosphorylated cTnI or dephosphorylated cTnI in complex with other troponin components. ABD13526 does not cross react with phosphorylated cTnI or with skeletal TnI.|
|Preservative & Stabilisers||0.09%Sodium Azide|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Human Troponin I (Cardiac/Dephosphorylated) Antibody, clone 22B11 is for research use only and not for use in diagnostic or therapeutic procedures.|
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