|Application ||WB, E|
|Preparation||Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBDNF. Anti-Human BDNF specific antibody was purified by affinity chromatography and then biotinylated.|
|WesternBlot||To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|Sandwich||To detect hBDNF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti-Human BDNF (60-080P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBDNF.|
|Direct||To detect hBDNF by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBDNF.|
|Formulation||A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Biotinylated Anti-Human BDNF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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