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Biotinylated Anti-Human BDNF Antibody

     
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Reactivity Human
Host Rabbit
Clonality Polyclonal
Additional Information
Preparation Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBDNF. Anti-Human BDNF specific antibody was purified by affinity chromatography and then biotinylated.
WesternBlot To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich To detect hBDNF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti-Human BDNF (60-080P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBDNF.
Direct To detect hBDNF by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBDNF.
Formulation A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage-20°C
PrecautionsBiotinylated Anti-Human BDNF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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$ 275.00
$ 216.00
Cat# ABG10032
Size:
Quantity:
(40 western blots)
Availability: 5-7days
Bulk Size
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