Biotinylated Anti-Rat CNTF Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Reactivity | Rat |
Host | Rabbit |
Clonality | Polyclonal |
Preparation | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat CNTF. Anti-Rat CNTF specific antibody was purified by affinity chromatography and then biotinylated. |
---|---|
WesternBlot | To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Sandwich | To detect Rat CNTF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti-Rat CNTF (62-052P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat CNTF. |
Direct | To detect Rat CNTF by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat CNTF. |
Formulation | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. |
Storage | -20°C |
Precautions | Biotinylated Anti-Rat CNTF Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.