|Application ||WB, IHC, E|
|Preparation||Produced from sera of rabbits immunized with highly pure recombinant Human CTACK. Anti-Human CTACK specific antibody was purified by affinity chromatography employing an immobilized Human CTACK matrix.|
|WesternBlot||To detect Human CTACK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human CTACK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|Sandwich||To detect Human CTACK by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human CTACK (60-106BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human CTACK.|
|Immunohistochemistry||This antibody stained HeLa cells. The primary antibody was incubated at 3.0 µg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm.|
|Formulation||A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Anti-Human CTACK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.