Anti-Human CTACK Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, E |
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Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Preparation | Produced from sera of rabbits immunized with highly pure recombinant Human CTACK. Anti-Human CTACK specific antibody was purified by affinity chromatography employing an immobilized Human CTACK matrix. |
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WesternBlot | To detect Human CTACK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human CTACK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Sandwich | To detect Human CTACK by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human CTACK (60-106BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human CTACK. |
Immunohistochemistry | This antibody stained HeLa cells. The primary antibody was incubated at 3.0 µg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm. |
Formulation | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
Storage | -20°C |
Precautions | Anti-Human CTACK Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
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