|Application ||WB, IHC, E|
|Preparation||Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hEGF. Anti-Human EGF specific antibody was purified by affinity chromatography employing immobilized hEGF matrix.|
|WesternBlot||To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.|
|Sandwich||To detect hEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human EGF (60-115BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF.|
|Immunohistochemistry||This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 μg/ml – 0.25 μg/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.|
|Neutralization||To yield one-half maximal inhibition [ND50] of the biological activity of hEGF (25.0 pg/ml), a concentration of < 0.1 µg/ml of this antibody is required.|
|Formulation||A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Anti-Human EGF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.