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Biotinylated Anti-Rat GM-CSF Antibody

     
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Additional Information
Preparation Produced from sera of rabbits immunized with highly pure recombinant Rat GM-CSF. Anti-Rat GM-CSF specific antibody was purified by affinity chromatography and then biotinylated.
WesternBlot To detect Rat GM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Rat GM-CSF is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions. 
Sandwich To detect Rat GM-CSF by sandwich ELISA (using 100 ml/well) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti- Rat GM-CSF (62-058P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat GM-CSF.
Direct

To detect Rat GM-CSF by direct ELISA (using 100 ml/well) a concentration of approximately 1.0 μg/ml of this antibody is required.  This biotinylated polyclonal antibody allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat GM-CSF.

Formulation A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage-20°C
PrecautionsBiotinylated Anti-Rat GM-CSF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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citation

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$ 275.00
$ 216.00
Cat# ABG10144-U050
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