|Application ||WB, IHC, E|
|Preparation||Produced from sera of rabbits pre-immunized with highly pure recombinant Rat IFN-γ. Anti-Rat IFN-γ specific antibody was purified by affinity chromatography employing immobilized Rat IFN-γ matrix.|
|WesternBlot||To detect Rat IFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IFN-γ is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.|
|Sandwich||To detect Rat IFN-γ by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Rat IFN-γ (62-061BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IFN-γ.|
|Immunohistochemistry||This antibody stained colchicine injected rat brain (including the cortex and median eminence) tissue. The primary antibody was incubated at 0.25 mg/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™) reagent. Optimal concentrations and conditions may vary.|
|Formulation||A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Anti-Rat IFN-γ Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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