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Anti-Human IGF-II Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Human
Host Rabbit
Clonality Polyclonal
Additional Information
Preparation Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-II (human IGF-II ). Anti-Human IGF-II specific antibody was purified by affinity chromatography employing immobilized hhIGF-II matrix.
WesternBlot To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich To detect hIGF-II by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human IGF-II (60-187BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II.
Immunohistochemistry This antibody stained formalin-fixed, paraffin-embedded sections of normal human placenta. The recommended concentration ranges from 0.5 µg/ml-5.0 µg/ml. An HRP-labeled polymer detection system was used with DAB chromogen. Recommended antigen retrievals are heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer or enzyme induced antigen retrieval with Proteinase K. Optimal concentrations and conditions may vary.
Neutralization To yield one-half maximal inhibition [ND50] of the biological activity of hIGF-II (10 ng/ml), a concentration of 3.1 - 4.6 µg/ml of this antibody is required.
Formulation A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
PrecautionsAnti-Human IGF-II Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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$ 275.00
$ 216.00
Cat# ABG10186
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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