|Application ||WB, E|
|Preparation||Produced in Sprague Dawley rats using highly pure (>98%) recombinant murine IL-10 as the immunizing antigen. This IgG2A antibody was purified from cell culture by Protein G affinity chromatography.|
|WesternBlot||To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.|
|Sandwich||In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 1600 pg/ml of recombinant murine IL-10 when used with BioGems' biotinylated antigen affinity purified anti-murine IL-10 (61-010BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.|
|Neutralization||To yield one-half maximal inhibition [ND50] of the biological activity of Murine IL-10 (0.5 ng/ml), a concentration of 3.0-5.0 μg/ml of this antibody is required.|
|Formulation||A sterile filtered antibody solution was lyophilized from PBS.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Anti-Murine IL-10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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