Anti-Murine IL-10 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Reactivity | Mouse |
Host | Rat |
Clonality | Monoclonal |
Preparation | Produced in Sprague Dawley rats using highly pure (>98%) recombinant murine IL-10 as the immunizing antigen. This IgG2A antibody was purified from cell culture by Protein G affinity chromatography. |
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WesternBlot | To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions. |
Sandwich | In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 1600 pg/ml of recombinant murine IL-10 when used with BioGems' biotinylated antigen affinity purified anti-murine IL-10 (61-010BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml. |
Neutralization | To yield one-half maximal inhibition [ND50] of the biological activity of Murine IL-10 (0.5 ng/ml), a concentration of 3.0-5.0 μg/ml of this antibody is required. |
Formulation | A sterile filtered antibody solution was lyophilized from PBS. |
Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
Storage | -20°C |
Precautions | Anti-Murine IL-10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
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