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Anti-Murine IL-12 Antibody
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Application 
| WB, E |
|---|---|
| Reactivity | Mouse |
| Host | Goat |
| Clonality | Polyclonal |
| Preparation | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mIL-12. Anti-Murine IL-12 specific antibody was purified by affinity chromatography employing immobilized mIL-12 matrix. |
|---|---|
| WesternBlot | To detect mIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Sandwich | To detect mIL-12 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine IL-12 (61-012BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-12. |
| Neutralization | To yield one-half maximal inhibition [ND50] of the biological activity of mIL-12 (2.0 ng/ml), a concentration of 0.002 - 0.003 µg/ml of this antibody is required. |
| Formulation | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Storage | -20°C |
| Precautions | Anti-Murine IL-12 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Research Areas
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Application Protocols
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$ 275.00
$ 216.00
Cat# ABG10201
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