|Application ||WB, IHC, E|
|Preparation||Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-33 (human IL-33). Anti-Human IL-33 specific antibody was purified by affinity chromatography employing immobilized hIL-33 matrix.|
|WesternBlot||To detect hIL-33 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-33 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|Sandwich||To detect hIL-33 by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human IL-33 (60-033BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-33.|
|Immunohistochemistry||This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.125 μg/ml-0.25 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.|
|Neutralization||To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-33 (1.0 ng/ml), a concentration of 0.2-0.4 μg/ml of this antibody is required.|
|Formulation||A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Anti-Human IL-33 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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