|Application ||WB, E|
|Preparation||Produced in BALB/c x ICR F1 mice using highly pure (>98%) recombinant human IL-4 as the immunizing antigen. This IgG1K antibody was purified from ascites fluid by Protein A affinity chromatography.|
|WesternBlot||To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 0.25-1.0 ng/lane, under reducing or non-reducing conditions.|
|Sandwich||In a sandwich ELISA (assuming 100µl/well), a concentration of 0.5-1.0 µg/ml of this antibody will detect at least 2000 pg/ml of recombinant human IL-4 when used with BioGems' biotinylated antigen affinity purified anti-human IL-4 (60-004BT) as the detection antibody at a concentration of approximately 2.0-4.0 µg/ml.|
|Formulation||A sterile filtered antibody solution was lyophilized from PBS.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Anti-Human IL-4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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