Biotinylated Anti-Murine IL-5 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Reactivity | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Preparation | Produced from sera of rabbits immunized with highly pure recombinant Murine IL-5. Anti-Murine IL-5 specific antibody was purified by affinity chromatography and then biotinylated. |
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WesternBlot | To detect Murine IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant Murine IL-5 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions. |
Sandwich | To detect Murine IL-5 by sandwich ELISA (using 100 ml/well) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti- Murine IL-5 (61-005P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine IL-5. |
Direct | To detect Murine IL-5 by direct ELISA (using 100 ml/well) a concentration of approximately 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine IL-5. |
Formulation | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. |
Storage | -20°C |
Precautions | Biotinylated Anti-Murine IL-5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
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