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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Antibody Pairs   >   Anti-Rat IL-6 Antibody   

Anti-Rat IL-6 Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Rat
Host Goat
Clonality Polyclonal
Additional Information
Preparation Produced from sera of goats pre-immunized with highly pure recombinant Rat IL-6. Anti-Rat IL-6 specific antibody was purified by affinity chromatography employing immobilized Rat IL-6 matrix.
WesternBlot To detect Rat IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich To detect Rat IL-6 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Rat IL-6 (62-006GBT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-6.
Immunohistochemistry This antibody stained colchicine injected rat brain (cingulate cortex) tissue.  The primary antibody was incubated at 1.0 μg/ml overnight at 4˚C.  This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™) reagent.  Optimal concentrations and conditions may vary.   
Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm.
Neutralization To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-6 (0.1 ng/ml), a concentration of 0.12-0.18 µg/ml of this antibody is required.
Formulation A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
PrecautionsAnti-Rat IL-6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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$ 275.00
$ 216.00
Cat# ABG10294
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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