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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Antibody Pairs   >   Anti-Human IL-6 Antibody   

Anti-Human IL-6 Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Human
Host Mouse
Clonality Monoclonal
Additional Information
Preparation Produced in BALB/c mice using highly pure (>98%) recombinant human IL-6 as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
WesternBlot To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
Sandwich In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 500 pg/ml of recombinant human IL-6 when used with BioGems' biotinylated antigen affinity purified anti-human IL-6 (60-006BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
Immunohistochemistry This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.125 μg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

*Additional Immunostaining data available. Please contact Tech Support for information.
Formulation A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
PrecautionsAnti-Human IL-6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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$ 216.00
Cat# ABG10300
(40 western blots)
Availability: 5-7days
Bulk Size
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