Anti-Human Maspin Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, E |
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Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Preparation | Produced from sera of rabbits immunized with highly pure recombinant Human Maspin. Anti-Human Maspin specific antibody was purified by affinity chromatography employing an immobilized Human Maspin matrix. |
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WesternBlot | To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Sandwich | To detect hMaspin by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human Maspin (60-210BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMaspin. |
Immunohistochemistry | This antibody stained formalin-fixed paraffin-embedded sections of human normal breast and human breast lobular carcinoma in situ. The recommended concentration is 7.8 ng/mL- 15.625 ng/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary. |
Formulation | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
Storage | -20°C |
Precautions | Anti-Human Maspin Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
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