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Anti-Human MCP-3 Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Human
Host Mouse
Clonality Monoclonal
Additional Information
Preparation Produced in C3H/HeJ mice using highly pure (>98%) recombinant human MCP-3 as the immunizing antigen. This IgG1K antibody was purified from ascites fluid by Protein A affinity chromatography.
WesternBlot To detect hMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-3 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Sandwich In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect recombinant human MCP-3 when used with BioGems' biotinylated antigen affinity purified anti-human MCP-3 (60-214BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
Neutralization To yield one-half maximal inhibition [ND50] of the biological activity of Human MCP-3(100 ng/ml), a concentration of 1.0-3.0 μg/ml of this antibody is required.
Formulation A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
PrecautionsAnti-Human MCP-3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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$ 216.00
Cat# ABG10368
(40 western blots)
Availability: 5-7days
Bulk Size
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