|Application ||WB, E|
|Preparation||Produced in BALB/c mice using highly pure recombinant human MIP-1α as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein G affinity chromatography.|
|WesternBlot||To detect Human MIP-1α by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-1α is 1.0-2.0 ng/lane, under non-reducing conditions.|
|Sandwich||In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 2000 pg/ml of recombinant human MIP-1α when used with BioGems' biotinylated antigen affinity purified anti-human MIP-1α (60-224BT) as the detection antibody at a concentration of approximately 1.0-2.0 µg/ml.|
|Neutralization||To yield one-half maximal inhibition [ND50] of the biological activity of Human MIP-1α (100 ng/ml), a concentration of 5.0-10.0 μg/ml of this antibody is required.|
|Formulation||A sterile filtered antibody solution was lyophilized from PBS.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Anti-Human MIP-1α Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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