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Anti-Human RANTES Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Human
Host Mouse
Clonality Monoclonal
Additional Information
Preparation Produced in BALB/c mice using highly pure (>98%) human RANTES as the immunizing antigen. This IgG2bK antibody was purified from ascites fluid by Protein A affinity chromatography.
WesternBlot To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Sandwich In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 500 pg/ml of recombinant human RANTES when used with BioGems' biotinylated antigen affinity purified anti-human RANTES (60-285BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
Immunohistochemistry This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 μg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 citrate buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.

*Additional Immunostaining data available. Please contact Tech Support for information.
Neutralization To yield one-half maximal inhibition [ND50] of the biological activity of Human RANTES (100 ng/ml), a concentration of 3.0-5.0 μg/ml of this antibody is required.
Formulation A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
PrecautionsAnti-Human RANTES Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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$ 216.00
Cat# ABG10473
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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